Home LiteratureArticle Details

PMID: 8670121 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid reduction of nitric oxide by mitochondria, and reversible inhibition of mitochondrial respiration by nitric oxide.

The Biochemical journal ·Vol. 315 ( Pt 1) ·1996-04-01 ·Pages 295-9

Borutaité V, Brown GC

Abstract

Nitric oxide (NO) inhibited the respiration rate of mitochondria isolated from rat heart at sub-micromolar concentrations of NO. The inhibition was rapidly and completely reversible, indicating that NO does not damage mitochondria. The sensitivity of respiration to NO depended on the oxygen concentration, substrate type and respiratory state of the mitochondria, consistent with NO competing with oxygen at cytochrome oxidase. Mitochondria catalysed a rapid rate of NO breakdown, which was greater in the absence of oxygen and was partly inhibited by cyanide and azide, suggesting that at least part of the NO breakdown was due to reduction of NO by cytochrome oxidase. The rapid rate of this breakdown suggests that mitochondrial breakdown of NO may be significant physiologically.

MeSH Terms

Animals Electrodes Electron Transport Complex IV/metabolism,pharmacology Mitochondria, Heart/drug effects,metabolism Nitric Oxide/metabolism,pharmacology Oxidation-Reduction Oxygen/pharmacology Oxygen Consumption/drug effects Rats Rats, Wistar

Chemicals

Nitric Oxide Electron Transport Complex IV Oxygen

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Borutaité V

Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.

Brown G C

References (26)

26 references, click to expand

Nitric oxide: a physiologic messenger molecule.
Annu Rev Biochem. 1994;63:175-95 PMID: 7526779
Nitric oxide potently and reversibly deenergizes mitochondria at low oxygen tension.
Biochem Biophys Res Commun. 1994 Oct 14;204(1):169-75 PMID: 7945356
Nitric oxide kills hepatocytes by mobilizing mitochondrial calcium.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1143-50 PMID: 7802643
Nanomolar concentrations of nitric oxide reversibly inhibit synaptosomal respiration by competing with oxygen at cytochrome oxidase.
FEBS Lett. 1994 Dec 19;356(2-3):295-8 PMID: 7805858
Role of nitric oxide in parasympathetic modulation of beta-adrenergic myocardial contractility in normal dogs.
J Clin Invest. 1995 Jan;95(1):360-6 PMID: 7529262
Direct measurement of the accumulation and mitochondrial conversion of nitric oxide within Chinese hamster ovary cells using an intracellular electron paramagnetic resonance technique.
Biochim Biophys Acta. 1995 Apr 13;1243(3):496-502 PMID: 7727525
Nitric oxide-dependent parasympathetic signaling is due to activation of constitutive endothelial (type III) nitric oxide synthase in cardiac myocytes.
J Biol Chem. 1995 Jun 16;270(24):14582-6 PMID: 7540173
Endothelial type nitric oxide synthase in skeletal muscle fibers: mitochondrial relationships.
Biochem Biophys Res Commun. 1995 Jun 15;211(2):375-81 PMID: 7540837
The interactions between nitric oxide and brain nerve terminals as studied by electron paramagnetic resonance.
Biochem Biophys Res Commun. 1995 Jul 17;212(2):404-12 PMID: 7626054
Nitric oxide regulates mitochondrial respiration and cell functions by inhibiting cytochrome oxidase.
FEBS Lett. 1995 Aug 7;369(2-3):136-9 PMID: 7649245
Reversible binding and inhibition of catalase by nitric oxide.
Eur J Biochem. 1995 Aug 15;232(1):188-91 PMID: 7556149
Nitric oxide produced by activated astrocytes rapidly and reversibly inhibits cellular respiration.
Neurosci Lett. 1995 Jul 7;193(3):201-4 PMID: 7478183
The localization of mitochondrial creatine kinase, and its use for the determination of the sidedness of submitochondrial particles.
Biochim Biophys Acta. 1973 Feb 16;291(3):764-73 PMID: 4696412
Reactions of nitric oxide with cytochrome c oxidase.
Biochemistry. 1980 Nov 11;19(23):5275-85 PMID: 6255988
Quantification of the contribution of various steps to the control of mitochondrial respiration.
J Biol Chem. 1982 Mar 25;257(6):2754-7 PMID: 7061448
Control of coronary vascular tone by nitric oxide.
Circ Res. 1990 Jun;66(6):1561-75 PMID: 2160870
Role of L-arginine-nitric oxide pathway in myocardial reoxygenation injury.
Am J Physiol. 1992 Feb;262(2 Pt 2):H616-20 PMID: 1539723
Negative inotropic effects of cytokines on the heart mediated by nitric oxide.
Science. 1992 Jul 17;257(5068):387-9 PMID: 1631560
Nitric oxide as a secretory product of mammalian cells.
FASEB J. 1992 Sep;6(12):3051-64 PMID: 1381691
Nitric oxide as a signal in blood vessels.
Trends Biochem Sci. 1992 Oct;17(10):399-402 PMID: 1280869
Control of cardiac muscle cell function by an endogenous nitric oxide signaling system.
Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):347-51 PMID: 7678347
The L-arginine-nitric oxide pathway.
N Engl J Med. 1993 Dec 30;329(27):2002-12 PMID: 7504210
Cytochrome oxidase evolved by tinkering with denitrification enzymes.
FEBS Lett. 1994 Mar 14;341(1):1-4 PMID: 8137905
Mechanisms of immune-mediated myocyte injury.
Circulation. 1994 May;89(5):2421-32 PMID: 8181167
Reversible inhibition of cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, by nitric oxide. Implications for neurodegenerative diseases.
FEBS Lett. 1994 May 23;345(1):50-4 PMID: 8194600
Nitric oxide in skeletal muscle.
Nature. 1994 Dec 8;372(6506):546-8 PMID: 7527495

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj3150295

Published

1996-04-01

Pages

295-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1217185

Subset

Grants

Wellcome Trust · United Kingdom

External Links

PubMed source DOI: 10.1042/bj3150295 PMC Full Text View journal details

Analysis Services

Rapid reduction of nitric oxide by mitochondria, and reversible inhibition of mitochondrial respiration by nitric oxide.

Contact

WeChat Official Account