Home LiteratureArticle Details

PMID: 8654377 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants.

The EMBO journal ·Vol. 15 ·No. 11 ·1996-06-03 ·Pages 2802-9

Allison LA, Simon LD, Maliga P

Abstract

The plastid genome in higher plants encodes subunits of an Escherichia coli-like RNA polymerase which initiates transcription of plastid genes from sequences resembling E.coli sigma70-type promoters. By deleting the gene for the essential beta subunit of the tobacco E.coli-like RNA polymerase, we have established the existence of a second plastid transcription system which does not utilize E.coli-like promoters. In contrast to the E.coli-like RNA polymerase, the novel transcription machinery preferentially transcribes genetic system genes rather than photosynthetic genes. Although the mutant plants are photosynthetically defective, transcription by this polymerase is sufficient for plastid maintenance and plant development.

MeSH Terms

Base Sequence DNA-Directed RNA Polymerases/genetics Gene Expression Regulation, Plant Molecular Sequence Data Photosynthesis Plants, Toxic Plastids/enzymology Promoter Regions, Genetic RNA, Ribosomal, 16S/genetics Sequence Deletion Sigma Factor/genetics Tobacco/genetics Transcription, Genetic

Chemicals

RNA, Ribosomal, 16S Sigma Factor DNA-Directed RNA Polymerases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Allison L A

Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, NJ 08855-0759, USA.

Simon L D

Maliga P

References (34)

34 references, click to expand

Plastid translation and transcription genes in a non-photosynthetic plant: intact, missing and pseudo genes.
EMBO J. 1991 Nov;10(11):3281-8 PMID: 1915295
Activity of the Chlamydomonas chloroplast rbcL gene promoter is enhanced by a remote sequence element.
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10819-23 PMID: 7971968
Inefficient rpl2 splicing in barley mutants with ribosome-deficient plastids.
Plant Cell. 1994 Oct;6(10):1455-65 PMID: 7994178
In vitro synthesis and processing of a maize chloroplast transcript encoded by the ribulose 1,5-bisphosphate carboxylase large subunit gene.
Mol Cell Biol. 1985 Oct;5(10):2733-45 PMID: 2874479
In vitro analysis of the pea chloroplast 16S rRNA gene promoter.
Mol Cell Biol. 1989 Dec;9(12):5650-9 PMID: 2586529
Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VIII. Localisation of a region involved in promoter selectivity.
Mol Gen Genet. 1986 Jun;203(3):487-91 PMID: 3528751
Structure and expression of a split chloroplast gene from mustard (Sinapis alba): ribosomal protein gene rps16 reveals unusual transcriptional features and complex RNA maturation.
Curr Genet. 1989 Jan;15(1):63-70 PMID: 2545357
Combination of in vitro capping and ribonuclease protection improves the detection of transcription start sites in chloroplasts.
Plant Mol Biol. 1992 May;19(2):309-11 PMID: 1623181
The 110-kDa polypeptide of spinach plastid DNA-dependent RNA polymerase: single-subunit enzyme or catalytic core of multimeric enzyme complexes?
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5509-13 PMID: 8516293
Stable transformation of plastids in higher plants.
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8526-30 PMID: 11607112
Characterization of a protein binding sequence in the promoter region of the 16S rRNA gene of the spinach chloroplast genome.
Nucleic Acids Res. 1991 Jul 11;19(13):3577-81 PMID: 1852607
The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.
Gene. 1982 Nov;20(1):91-102 PMID: 7160620
Separation of two classes of plastid DNA-dependent RNA polymerases that are differentially expressed in mustard (Sinapis alba L.) seedlings.
Plant Mol Biol. 1994 Apr;25(1):69-81 PMID: 8003698
Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice.
Mol Gen Genet. 1993 Jan;236(2-3):341-6 PMID: 8437578
High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):913-7 PMID: 8381537
Translation of psbA mRNA is regulated by light via the 5'-untranslated region in tobacco plastids.
Plant J. 1994 Oct;6(4):547-53 PMID: 7987413
Chloroplast rpoA, rpoB, and rpoC genes specify at least three components of a chloroplast DNA-dependent RNA polymerase active in tRNA and mRNA transcription.
J Biol Chem. 1988 Oct 5;263(28):14302-7 PMID: 3049574
Regulation of rDNA transcription in chloroplasts: promoter exclusion by constitutive repression.
Genes Dev. 1994 Dec 1;8(23):2928-38 PMID: 7995527
Transfer of rpl22 to the nucleus greatly preceded its loss from the chloroplast and involved the gain of an intron.
EMBO J. 1991 Oct;10(10):3073-8 PMID: 1915281
Chlamydomonas reinhardtii as the photosynthetic yeast.
Annu Rev Genet. 1995;29:209-30 PMID: 8825474
Plastid Genes Encoding the Transcription/Translation Apparatus Are Differentially Transcribed Early in Barley (Hordeum vulgare) Chloroplast Development (Evidence for Selective Stabilization of psbA mRNA).
Plant Physiol. 1993 Mar;101(3):781-791 PMID: 12231729
A novel light-regulated promoter is conserved in cereal and dicot chloroplasts.
Plant Cell. 1992 Jul;4(7):785-98 PMID: 1392595
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.
EMBO J. 1986 Sep;5(9):2043-2049 PMID: 16453699
Genetic mosaics and cell lineage analysis in plants.
Trends Genet. 1989 Aug;5(8):273-7 PMID: 2686117
Light-responsive and transcription-enhancing elements regulate the plastid psbD core promoter.
EMBO J. 1995 Aug 1;14(15):3721-30 PMID: 7641691
Two types of chloroplast gene promoters in Chlamydomonas reinhardtii.
Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3453-7 PMID: 1565638
A methyl jasmonate-induced shift in the length of the 5' untranslated region impairs translation of the plastid rbcL transcript in barley.
EMBO J. 1993 Apr;12(4):1505-12 PMID: 8467800
Chloroplast rRNA transcription from structurally different tandem promoters: an additional novel-type promoter.
Curr Genet. 1995 Feb;27(3):280-4 PMID: 7736614
An additional promoter within the protein-coding region of the psbD-psbC gene cluster in tobacco chloroplast DNA.
Nucleic Acids Res. 1989 Dec 11;17(23):9583-91 PMID: 2481263
Chloroplast rps15 and the rpoB/C1/C2 gene cluster are strongly transcribed in ribosome-deficient plastids: evidence for a functioning non-chloroplast-encoded RNA polymerase.
EMBO J. 1993 Feb;12(2):563-71 PMID: 8440246
Dynamic regulation of chloroplast transcription.
Plant Physiol. 1993 Oct;103(2):309-13 PMID: 8029331
Evolutionary transfer of the chloroplast tufA gene to the nucleus.
Nature. 1990 Mar 15;344(6263):262-5 PMID: 2314461
A subpopulation of spinach chloroplast tRNA genes does not require upstream promoter elements for transcription.
Nucleic Acids Res. 1986 Oct 10;14(19):7541-56 PMID: 3774537
Identification of a sequence-specific DNA binding factor required for transcription of the barley chloroplast blue light-responsive psbD-psbC promoter.
Plant Cell. 1995 Sep;7(9):1445-57 PMID: 8589628

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1996-06-03

Pages

2802-9

Language

English

Region

England

NLM ID

8208664

PMCID

PMC450217

Subset

External Links

PubMed source PMC Full Text View journal details

Analysis Services

Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants.

Contact

WeChat Official Account