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PMID: 8649416 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Determinants of Drosophila fushi tarazu mRNA instability.

Molecular and cellular biology ·Vol. 16 ·No. 6 ·1996-06-00 ·Pages 3047-53

Riedl A, Jacobs-Lorena M

Abstract

The fushi tarazu gene is essential for the establishment of the Drosophila embryonic body plan. When first expressed in early embryogenesis, fushi tarazu mRNA is uniformly distributed over most of the embryo. Subsequently, fushi tarazu mRNA expression rapidly evolves into a pattern of seven stripes that encircle the embryo. The instability of fushi tarazu mRNA is probably crucial for attaining this localized pattern of expression. mRNA stability in transgenic embryos was measured by a new method that does not use drugs or external interference. Experiments using hybrid genes that fuse fushi tarazu sequences to those of the stable ribosomal protein A1 mRNA provide evidence for at least two destabilizing elements in the fushi tarazu mRNA, one located within the 5' one-third of the mRNA and the other near the 3' end (termed FIE3 for ftz instability element 3'). The FIE3 lies within a 201-nucleotide sequence just upstream of the polyadenylation signal and can act autonomously to destabilize a heterologous mRNA. Further deletion constructs identified an essential 68-nucleotide element within the FIE3. Lack of homology between this element and other previously identified destabilization sequences suggests that FIE3 contains a novel RNA destabilization element.

MeSH Terms
Animals Animals, Genetically Modified Base Sequence DNA Primers/genetics Drosophila/embryology,genetics,metabolism Drosophila Proteins Fushi Tarazu Transcription Factors Gene Expression Regulation, Developmental Genes, Insect Homeodomain Proteins/genetics Models, Biological Molecular Sequence Data Nucleic Acid Conformation RNA, Messenger/chemistry,genetics,metabolism Sequence Deletion Sequence Homology, Nucleic Acid
Chemicals
DNA Primers Drosophila Proteins Fushi Tarazu Transcription Factors Homeodomain Proteins RNA, Messenger ftz protein, Drosophila
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Riedl A
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4955, USA.
Jacobs-Lorena M
References (40)
40 references, click to expand
  1. U-rich sequence-binding proteins (URBPs) interacting with a 20-nucleotide U-rich sequence in the 3' untranslated region of c-fos mRNA may be involved in the first step of c-fos mRNA degradation.
    Mol Cell Biol. 1992 Jul;12(7):2931-40 PMID: 1620106
  2. P transposons controlled by the heat shock promoter.
    Mol Cell Biol. 1986 May;6(5):1640-9 PMID: 3023899
  3. Staufen protein associates with the 3'UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner.
    Cell. 1994 Dec 30;79(7):1221-32 PMID: 8001156
  4. Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential.
    Cell. 1995 May 5;81(3):403-12 PMID: 7736592
  5. Postinduction turnoff of beta-interferon gene expression.
    Mol Cell Biol. 1990 Apr;10(4):1329-37 PMID: 2157136
  6. Regulation of interaction of the iron-responsive element binding protein with iron-responsive RNA elements.
    Mol Cell Biol. 1989 Nov;9(11):5055-61 PMID: 2601708
  7. Predicting optimal and suboptimal secondary structure for RNA.
    Methods Enzymol. 1990;183:281-306 PMID: 1690335
  8. Genetic transformation of Drosophila with transposable element vectors.
    Science. 1982 Oct 22;218(4570):348-53 PMID: 6289436
  9. Apical localization of pair-rule transcripts requires 3' sequences and limits protein diffusion in the Drosophila blastoderm embryo.
    Cell. 1991 Nov 29;67(5):927-40 PMID: 1959136
  10. Identification of an activity that interacts with the 3'-untranslated region of c-myc mRNA and the role of its target sequence in mediating rapid mRNA degradation.
    J Biol Chem. 1994 Feb 11;269(6):4532-8 PMID: 8308023
  11. Analysis of chimeric mRNAs derived from the STE3 mRNA identifies multiple regions within yeast mRNAs that modulate mRNA decay.
    Nucleic Acids Res. 1992 Oct 25;20(20):5365-73 PMID: 1437553
  12. Mutations affecting the stability of the fushi tarazu protein of Drosophila.
    Genes Dev. 1990 Nov;4(11):1936-50 PMID: 2276626
  13. Molecular evolution among some Drosophila species groups as indicated by two-dimensional electrophoresis.
    J Mol Evol. 1988;27(3):250-60 PMID: 3138427
  14. Cis-acting sequences in the 5'-untranslated region of the ribosomal protein A1 mRNA mediate its translational regulation during early embryogenesis of Drosophila.
    J Biol Chem. 1992 Jan 15;267(2):1159-64 PMID: 1730640
  15. Structural analysis of the Drosophila rpA1 gene, a member of the eucaryotic 'A' type ribosomal protein family.
    Nucleic Acids Res. 1987 Feb 11;15(3):987-1003 PMID: 3103101
  16. Identification of an AUUUA-specific messenger RNA binding protein.
    Science. 1989 Nov 3;246(4930):664-6 PMID: 2814487
  17. Control elements of the Drosophila segmentation gene fushi tarazu.
    Cell. 1985 Dec;43(3 Pt 2):603-13 PMID: 3935327
  18. Improved predictions of secondary structures for RNA.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7706-10 PMID: 2479010
  19. Regulation of mRNA stability in development.
    Annu Rev Genet. 1994;28:263-82 PMID: 7893126
  20. A coding region segment is necessary, but not sufficient for rapid decay of the HIS3 mRNA in yeast.
    Gene. 1992 May 1;114(1):35-41 PMID: 1587483
  21. Studies of nuclear and cytoplasmic behaviour during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis.
    J Cell Sci. 1983 May;61:31-70 PMID: 6411748
  22. On finding all suboptimal foldings of an RNA molecule.
    Science. 1989 Apr 7;244(4900):48-52 PMID: 2468181
  23. Ultrastructural patterns of RNA synthesis during early embryogenesis of Drosophila melanogaster.
    Cell. 1976 Jun;8(2):305-19 PMID: 822943
  24. Translational regulation of a specific gene during oogenesis and embryogenesis of Drosophila.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3359-63 PMID: 16593324
  25. Vectors for P-mediated transformation in Drosophila.
    Biotechnology. 1988;10:437-56 PMID: 2850048
  26. Hypoxia up-regulates the activity of a novel erythropoietin mRNA binding protein.
    J Biol Chem. 1991 Sep 5;266(25):16594-8 PMID: 1653242
  27. Patterns of engrailed and fushi tarazu transcripts reveal novel intermediate stages in Drosophila segmentation.
    Nature. 1985 Dec 5-11;318(6045):433-9 PMID: 4069216
  28. The sequence of the stem and flanking sequences at the 3' end of histone mRNA are critical determinants for the binding of the stem-loop binding protein.
    Nucleic Acids Res. 1995 Feb 25;23(4):654-62 PMID: 7899087
  29. A molecular analysis of fushi tarazu, a gene in Drosophila melanogaster that encodes a product affecting embryonic segment number and cell fate.
    Cell. 1984 Jul;37(3):843-51 PMID: 6430569
  30. Repression and turnover pattern fushi tarazu RNA in the early Drosophila embryo.
    Cell. 1986 Dec 5;47(5):747-54 PMID: 3096577
  31. Translational regulation of mRNAs for ribosomal proteins during early Drosophila development.
    Biochemistry. 1985 Oct 8;24(21):5798-803 PMID: 3936537
  32. Rates of synthesis of major classes of RNA in Drosophila embryos.
    Dev Biol. 1979 May;70(1):217-31 PMID: 110635
  33. Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster.
    Mol Cell Biol. 1985 Dec;5(12):3583-92 PMID: 3939320
  34. The basis for a heat-induced developmental defect: defining crucial lesions.
    Genes Dev. 1995 Sep 15;9(18):2240-50 PMID: 7557378
  35. Spatial distribution of transcripts from the segmentation gene fushi tarazu during Drosophila embryonic development.
    Cell. 1984 Jul;37(3):833-41 PMID: 6430568
  36. Non-periodic cues generate seven ftz stripes in the Drosophila embryo.
    Mech Dev. 1995 Apr;50(2-3):163-75 PMID: 7619728
  37. Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm.
    Genes Dev. 1987 Dec;1(10):1226-37 PMID: 3123317
  38. The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways.
    Genes Dev. 1989 Jan;3(1):60-72 PMID: 2496006
  39. The protein-coding region of c-myc mRNA contains a sequence that specifies rapid mRNA turnover and induction by protein synthesis inhibitors.
    Genes Dev. 1991 Feb;5(2):232-43 PMID: 1995415
  40. Interplay of two functionally and structurally distinct domains of the c-fos AU-rich element specifies its mRNA-destabilizing function.
    Mol Cell Biol. 1994 Jan;14(1):416-26 PMID: 7903419
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.6.3047
Published
1996-06-00
Pages
3047-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231299
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.6.3047 PMC Full Text View journal details
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