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PMID: 8645296 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new plasmid-encoded proteic killer gene system: cloning, sequencing, and analyzing hig locus of plasmid Rts1.

Biochemical and biophysical research communications ·Vol. 220 ·No. 2 ·1996-03-18 ·Pages 280-4

Tian QB, Ohnishi M, Tabuchi A, Terawaki Y

Abstract

A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of a higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the higB function both in cis and in trans.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Escherichia coli/genetics Escherichia coli Proteins Molecular Sequence Data Phenotype Plasmids Polymerase Chain Reaction Recombinant Proteins Restriction Mapping Sequence Analysis, DNA Temperature

Chemicals

Bacterial Proteins Escherichia coli Proteins HigA protein, E coli HigB protein, E coli Recombinant Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tian Q B

Department of Bacteriology, Shinshu University School of Medicine, Japan.

Ohnishi M

Tabuchi A

Terawaki Y

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.1996.0396

Published

1996-03-18

Pages

280-4

Language

English

Region

United States

NLM ID

0372516

Subset

Databases

GENBANK

U43847

External Links

PubMed source DOI: 10.1006/bbrc.1996.0396 View journal details

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A new plasmid-encoded proteic killer gene system: cloning, sequencing, and analyzing hig locus of plasmid Rts1.

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