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PMID: 8642631 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The turnip yellow mosaic virus tRNA-like structure cannot be replaced by generic tRNA-like elements or by heterologous 3' untranslated regions known to enhance mRNA expression and stability.

Journal of virology ·Vol. 70 ·No. 4 ·1996-04-00 ·Pages 2107-15

Skuzeski JM, Bozarth CS, Dreher TW

Abstract

The tRNA-like structure (TLS) at the 3' end of the turnip yellow mosaic virus genome was replaced with heterologous tRNA-like elements, and with a poly(A) tail, in order to assess its role. Replacement with the valylatable TLSs from two closely related tymoviruses resulted in infectious viruses. In contrast, no systemic symptoms on plants, and only low viral accumulations in protoplasts, were observed for three chimeric genomes with 3' sequences known to enhance mRNA stability and translatability. One of these chimeras had a poly(A) tail, and the others had the TLS with associated upstream pseudoknot tracts from the 3' ends of brome mosaic and tobacco mosaic viruses. The latter two chimeric RNAs were shown to be appropriately folded by demonstrating their aminoacylation in vitro with tyrosine and histidine, respectively. The results show that enhancement of genome stability or gene expression is not the major role of the turnip yellow mosaic virus TLS. The major role is likely to be replicational, dependent on features present in tymoviral TLSs but not in generic tRNA-like structures.

MeSH Terms
Base Sequence Brassica/virology Genome, Plant Histidine/chemistry Molecular Sequence Data Nucleic Acid Conformation Poly A/chemistry,genetics RNA/chemistry,genetics RNA, Plant RNA, Transfer/chemistry,genetics RNA, Viral/chemistry,genetics Tymovirus/genetics Tyrosine/chemistry Valine/chemistry
Chemicals
RNA, Plant RNA, Viral RNA, recombinant Poly A Tyrosine Histidine RNA RNA, Transfer Valine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Skuzeski J M
Department of Agricultural Chemistry, Oregon State University, Corvalis, Oregon 97331-7301, USA.
Bozarth C S
Dreher T W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.4.2107-2115.1996
Published
1996-04-00
Pages
2107-15
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190047
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.4.2107-2115.1996 PMC Full Text View journal details
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