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PMID: 8642294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reversibility of T helper 1 and 2 populations is lost after long-term stimulation.

The Journal of experimental medicine ·Vol. 183 ·No. 3 ·1996-03-01 ·Pages 901-13

Murphy E, Shibuya K, Hosken N, Openshaw P, Maino V, Davis K, Murphy K, O'Garra A

Abstract

Commitment of T helper 1 (Th1) or Th2 populations developing during an immune response to a pathogen, or an inappropriate immune response to an allergen or autoantigen, may determine the difference between health and chronic disease. We show that strongly polarized Th1 and Th2 populations assessed by immunoassay are heterogeneous using flow cytometry to detect single cells producing interferon gamma (IFN-gamma) and interleukin 4 (IL-4). Th1 populations arising after 1 wk of stimulation in IL-12 plus anti-IL-4 antibodies could convert to Th2 cells when restimulated in IL-4. Th2 populations resulting from stimulation for 1 wk in IL-4 could give rise to Th1 cells upon restimulation in IL-12 plus anti-IL-4. In contrast, the cytokine profiles of long-term Th1 and Th2 populations arising originally from repeated stimulation in IL-12 or IL-4 appeared more homogeneous and were not reversible, although IL-4 dramatically reduced the number of IFN-gamma-producing Th1 cells. This may explain previous reports that Th1 cells can be converted to Th2 cells.

MeSH Terms
Allergens Animals Antigen-Presenting Cells/immunology Autoantigens CD4-Positive T-Lymphocytes/immunology Chronic Disease Clone Cells Flow Cytometry Humans Immunophenotyping Interferon-gamma/biosynthesis Interleukin-4/biosynthesis Lymphocyte Activation Mice Mice, Inbred BALB C Mice, Transgenic Models, Immunological Receptors, Antigen, T-Cell, alpha-beta/biosynthesis,genetics T-Lymphocyte Subsets/cytology,immunology T-Lymphocytes, Helper-Inducer/cytology,immunology Time Factors
Chemicals
Allergens Autoantigens Receptors, Antigen, T-Cell, alpha-beta Interleukin-4 Interferon-gamma
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Murphy E
DNAX Research Institute, Palo Alto, California 94301-1104, USA.
Shibuya K
Hosken N
Openshaw P
Maino V
Davis K
Murphy K
O'Garra A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.183.3.901
Published
1996-03-01
Pages
901-13
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192360
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1084/jem.183.3.901 PMC Full Text View journal details
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