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PMID: 8641770 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Susceptibility of Chlamydia trachomatis to protegrins and defensins.

Infection and immunity ·Vol. 64 ·No. 3 ·1996-03-00 ·Pages 709-13

Yasin B, Harwig SS, Lehrer RI, Wagar EA

Abstract

We compared the susceptibilities of Chlamydia trachomatis elementary bodies (EBs) to human defensin HNP-2 and porcine protegrin PG-1, cysteine-rich beta-sheet antimicrobial peptides produced by mammalian leukocytes. Although both peptides protected McCoy cell monolayers from infection by chlamydial EBs, protegrins were especially potent. Protegrin-mediated inactivation of chlamydiae occurred rapidly, was relatively independent of the presence of serum, and was effective against serovars L2, D, and H. Protegrin-treated EBs showed striking morphological changes, with obvious damage to their limiting membranes and loss of their cytoplasmic contents and nucleoid. Their effectiveness against chlamydial EBs and other sexually transmitted pathogens combined with their relative lack of cytotoxicity suggests that protegrins and related molecules could serve as prototypes for topical agents to prevent sexually transmitted chlamydial infection.

MeSH Terms
Animals Anti-Bacterial Agents/pharmacology Antimicrobial Cationic Peptides Blood Proteins/pharmacology Chlamydia trachomatis/drug effects Defensins Humans Microbial Sensitivity Tests Proteins/pharmacology Rabbits alpha-Defensins
Chemicals
Anti-Bacterial Agents Antimicrobial Cationic Peptides Blood Proteins Defensins Proteins alpha-Defensins human neutrophil peptide 2 protegrin-1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yasin B
Department of Pathology and Laboratory Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA.
Harwig S S
Lehrer R I
Wagar E A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.3.709-713.1996
Published
1996-03-00
Pages
709-13
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173826
Subset
IM
Grants
NIAID NIH HHS · AI 22839 · United States
NIAID NIH HHS · AI 37945 · United States
External Links
PubMed source DOI: 10.1128/iai.64.3.709-713.1996 PMC Full Text View journal details
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