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PMID: 8637918 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 6 ·1996-03-19 ·Pages 2588-92

Zhou LJ, Tedder TF

Abstract

Dendritic cells are potent antigen-presenting cells that initiate primary immune responses. Although dendritic cells derive from bone marrow stem cells, the intermediate stages in their development remain unknown. In this study, plastic-adherent blood monocytes (CD14+, CD1a-) cultured for 7 days with granulocyte-monocyte colony-stimulating factor, interleukin 4, and tumor necrosis factor alpha were shown to differentiate into CD1a+ CD83+ dendritic cells. These cells displayed all phenotypic and morphologic characteristics of mature dendritic cells and were the most potent stimulatory cells in allogeneic mixed leukocyte reactions. The identification of specific culture conditions that generate large numbers of dendritic cells from purified monocytes uncovers an important step in dendritic cell maturation that will allow the further characterization of their role in autoimmune diseases, graft rejection, and human immunodeficiency virus infection.

MeSH Terms
Adult Antigens, CD Cell Differentiation/drug effects Cells, Cultured Dendritic Cells/cytology,immunology Flow Cytometry Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology Humans Immunoglobulins Interleukin-4/pharmacology Lipopolysaccharide Receptors Lymphocyte Culture Test, Mixed Membrane Glycoproteins Monocytes/cytology,immunology Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Antigens, CD CD83 antigen Immunoglobulins Lipopolysaccharide Receptors Membrane Glycoproteins Tumor Necrosis Factor-alpha Interleukin-4 Granulocyte-Macrophage Colony-Stimulating Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhou L J
Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
Tedder T F
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.6.2588
Published
1996-03-19
Pages
2588-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39841
Subset
IM
Grants
NIAID NIH HHS · AI-26872 · United States
NCI NIH HHS · CA-54464 · United States
NHLBI NIH HHS · HL-50985 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.6.2588 PMC Full Text View journal details
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