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PMID: 8636216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Onset of re-epithelialization after skin injury correlates with a reorganization of keratin filaments in wound edge keratinocytes: defining a potential role for keratin 16.

The Journal of cell biology ·Vol. 132 ·No. 3 ·1996-02-00 ·Pages 381-97

Paladini RD, Takahashi K, Bravo NS, Coulombe PA

Abstract

Injury to stratified epithelia causes a strong induction of keratins 6 (K6) and 16 (K16) in post-mitotic keratinocytes located at the wound edge. We show that induction of K6 and K16 occurs within 6 h after injury to human epidermis. Their subsequent accumulation in keratinocytes correlates with the profound reorganization of keratin filaments from a pan-cytoplasmic distribution to one in which filaments are aggregated in a juxtanuclear location, opposite to the direction of cell migration. This filament reorganization coincides with additional cytoarchitectural changes and the onset of re-epithelialization after 18 h post-injury. By following the assembly of K6 and K16 in vitro and in cultured cells, we find that relative to K5 and K14, a well-characterized keratin pair that is constitutively expressed in epidermis, K6 and K16 polymerize into short 10-nm filaments that accumulate near the nucleus, a property arising from K16. Forced expression of human K16 in skin keratinocytes of transgenic mice causes a retraction of keratin filaments from the cell periphery, often in a polarized fashion. These results imply that K16 may not have a primary structural function akin to epidermal keratins. Rather, they suggest that in the context of epidermal wound healing, the function of K16 could be to promote a reorganization of the cytoplasmic array of keratin filaments, an event that precedes the onset of keratinocyte migration into the wound site.

MeSH Terms
Adult Animals Cells, Cultured Cloning, Molecular DNA, Complementary Epidermis/pathology,physiology Epithelium/physiology Escherichia coli Fibroblasts/cytology,physiology Gene Expression Humans Immune Sera Keratinocytes/pathology,physiology Keratins/biosynthesis,physiology,ultrastructure Mice Mice, Transgenic Microscopy, Electron Recombinant Proteins/biosynthesis,isolation & purification,ultrastructure Skin/injuries,pathology Skin Physiological Phenomena Wound Healing
Chemicals
DNA, Complementary Immune Sera Recombinant Proteins Keratins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Paladini R D
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Takahashi K
Bravo N S
Coulombe P A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.132.3.381
Published
1996-02-00
Pages
381-97
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120730
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.132.3.381 PMC Full Text View journal details
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