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PMID: 8635477 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification of a subdomain within DNA-(cytosine-C5)-methyltransferases responsible for the recognition of the 5' part of their DNA target.

The EMBO journal ·Vol. 15 ·No. 6 ·1996-03-15 ·Pages 1443-50

Lange C, Wild C, Trautner TA

Abstract

In previous work on DNA-(cytosine-C5)-methyltransferases (C5-MTases), domains had been identified which are responsible for the sequence specificity of the different enzymes (target-recognizing domains, TRDs). Here we have analyzed the DNA methylation patterns of two C5-MTases containing reciprocal chimeric TRDs, consisting of the N- and C-terminal parts derived from two different parental TRDs specifying the recognition of 5'-CC(A/T)GG-3' and 5'-GCNGC-3'. Sequences recognized by these engineered MTases were non-symmetrical and degenerate, but contained at their 5' part a consensus sequence which was very similar to the 5' part of the target recognized by the parental TRD which contributed the N-terminal moiety of the chimeric TRD. The results are discussed in connection with the present understanding of the mechanism of DNA target recognition by C5-MTases. They demonstrate the possibility of designing C5-MTases with novel DNA methylation specificities.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites DNA/metabolism DNA-Cytosine Methylases/genetics,metabolism Methylation Molecular Sequence Data Mutagenesis, Site-Directed Protein Binding Recombinant Fusion Proteins/metabolism Sequence Homology, Amino Acid Structure-Activity Relationship Substrate Specificity
Chemicals
Recombinant Fusion Proteins DNA DNA-Cytosine Methylases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lange C
Max-Planck-Institut für molekulare Genetik, Berlin, Germany.
Wild C
Trautner T A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-03-15
Pages
1443-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450049
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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