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PMID: 8627762 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Open reading frame 5 of porcine reproductive and respiratory syndrome virus as a cause of virus-induced apoptosis.

Journal of virology ·Vol. 70 ·No. 5 ·1996-05-00 ·Pages 2876-82

Suárez P, Díaz-Guerra M, Prieto C, Esteban M, Castro JM, Nieto A, Ortín J

Abstract

The gene product of open reading frame 5 (p25) of porcine reproductive and respiratory syndrome (PRRS) virus has been expressed by coinfection of culture cells with vaccinia virus expressing the T7 RNA polymerase and a recombinant vaccinia virus encoding the open reading frame 5 gene under the T7 promoter and the encephalomyocarditis virus internal ribosome entry site. In spite of the reported efficiency of the expression system, very poor accumulation of p25 protein was observed and a strong cytotoxicity was produced in the doubly infected cells. This cell toxicity was shown to occur by induction of apoptosis, as indicated by nucleosome ladder formation, chromatin condensation, and rRNA degradation. Apoptosis induction was also observed after infection of cultured cells with an adapted PRRS virus strain and after infection of swine macrophage cells with a PRRS virus field strain. Contrary to the observations made for other cases of virus-induced apoptosis, we could not prevent p25 protein-induced apoptosis by using a cell line permanently expressing Bcl-2 protein.

MeSH Terms
Animals Apoptosis Arterivirus/genetics,isolation & purification,physiology Base Sequence Cell Line Chlorocebus aethiops Cloning, Molecular DNA, Viral/analysis HeLa Cells Humans Molecular Sequence Data Open Reading Frames Plasmids RNA, Viral/biosynthesis,isolation & purification Restriction Mapping Spain Swine Swine Diseases/virology Syndrome Vaccinia virus Viral Envelope Proteins Viral Proteins/biosynthesis
Chemicals
DNA, Viral RNA, Viral Viral Envelope Proteins Viral Proteins glycoprotein 5, PRRSV
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Suárez P
Centro Nacional de Biotecnologiá, Madrid, Spain.
Díaz-Guerra M
Prieto C
Esteban M
Castro J M
Nieto A
Ortín J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.5.2876-2882.1996
Published
1996-05-00
Pages
2876-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190145
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.5.2876-2882.1996 PMC Full Text View journal details
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