Consistent risk group-associated differences in human immunodeficiency virus type 1 vpr, vpu and V3 sequences despite independent evolution.

The Journal of general virology ·Vol. 77 ( Pt 4) ·1996-04-00 ·Pages 783-92

Kuiken CL, Cornelissen MT, Zorgdrager F, Hartman S, Gibbs AJ, Goudsmit J

Abstract

Human immunodeficiency virus 1 type vpr, vpu and V3 sequences from 15 homosexual men and 19 intravenous drug users in the Amsterdam Cohort studies were analysed. Previously, we reported that V3 domains of viruses from drug users are distinguishable from those of homosexual men on the basis of two silent mutations. Phylogenetic analysis of vpr, vpu and V3 shows that differences in all three regions correlate with risk group. Two positions in both vpr and vpu were found to differ significantly between the risk groups. The distinguishing positions were confirmed for sequences from 11 Scottish and four German samples. The three regions show relatively independent evolution patterns; they resulted in different phylogenies, the only stable clustering being that based on the risk group distinction. Pairwise differences between sequences of the genes were moderately correlated (around 0.30). Surprisingly, when only silent changes are counted, the correlations dropped almost to zero, indicating that the evolution towards independence was more advanced in the silent than in the non-silent positions. This suggests that selection at the amino acid level is not the primary driving force for the independent evolutionary behaviour of the genes. Recombination, combined with restrictions on certain amino acids because of epistatic interactions between the genes, could be an alternative explanation of this phenomenon.

MeSH Terms

Base Sequence Cohort Studies DNA, Viral Europe/epidemiology Evolution, Molecular Gene Products, vpr/genetics Genes, Viral Genetic Variation HIV Envelope Protein gp120/genetics HIV Infections/epidemiology,virology HIV-1/genetics Homosexuality Human Immunodeficiency Virus Proteins Humans Male Molecular Sequence Data Peptide Fragments/genetics Phylogeny Risk Factors Sequence Homology, Nucleic Acid Substance Abuse, Intravenous/complications Viral Regulatory and Accessory Proteins/genetics vpr Gene Products, Human Immunodeficiency Virus

Chemicals

DNA, Viral Gene Products, vpr HIV Envelope Protein gp120 HIV envelope protein gp120 (305-321) Human Immunodeficiency Virus Proteins Peptide Fragments Viral Regulatory and Accessory Proteins vpr Gene Products, Human Immunodeficiency Virus vpu protein, Human immunodeficiency virus 1

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kuiken C L

Department of Human Retrovirology, University of Amersterdam, The Netherlands. kuiken@amc.uva.nl

Cornelissen M T

Zorgdrager F

Hartman S

Gibbs A J

Goudsmit J

Article Info

Journal

The Journal of general virology

Abbr.

J Gen Virol

ISSN

0022-1317

DOI

10.1099/0022-1317-77-4-783

kuiken@amc.uva.nl

Published

1996-04-00

Pages

783-92

Language

English

Region

England

NLM ID

0077340

Subset

Databases

GENBANK

Z29280, Z29296, Z29298, Z29299, Z29302, Z29303, Z29304, Z29306, Z29307, Z29310, Z29311, Z29312, Z29315, Z29319, Z29320, Z29321, Z29322, Z29323, Z29324, Z68033, Z68047, Z68062, Z68070, Z68074, Z68086, Z68508, Z68509, Z68510, Z68511, Z68512

External Links

PubMed source DOI: 10.1099/0022-1317-77-4-783 View journal details

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Consistent risk group-associated differences in human immunodeficiency virus type 1 vpr, vpu and V3 sequences despite independent evolution.

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