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PMID: 8626705 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A delayed-early response nuclear gene encoding MRPL12, the mitochondrial homologue to the bacterial translational regulator L7/L12 protein.

The Journal of biological chemistry ·Vol. 271 ·No. 19 ·1996-05-10 ·Pages 11468-76

Marty L, Fort P

Abstract

We have characterized a new delayed-early response mRNA encoding a 21-kDa product (MRPL12) that accumulates during the G1 phase of growth-stimulated cells. MRPL12 is the mammalian homologue to chloroplastic and bacterial L12 ribosomal proteins. Immunofluorescence microscopy and cell fractionation indicate a predominant mitochondrial localization in various mammalian cell lines. The NH2-terminal 49 amino acids are necessary and sufficient to target the protein within the mitochondria and are probably cleaved off during import. MRPL12 proteins associated in vitro and cofractionate with ribosomal structures, as is the case for prokaryotic L12 proteins. Expression of a dominant inhibitory truncated protein leads to a severe reduction in cell growth by inhibiting mitochondrial ATP production. MRPL12 is the first mammalian mitochondrial ribosomal protein to be characterized.

MeSH Terms

3T3 Cells Amino Acid Sequence Animals Bacterial Proteins/metabolism Base Sequence Cell Cycle Proteins Cell Division Cell Line Cell Nucleus Chlorocebus aethiops Chloroplasts/metabolism Cloning, Molecular Cricetinae DNA, Complementary Escherichia coli/metabolism Gene Expression Geobacillus stearothermophilus/metabolism Glutathione Transferase/biosynthesis HeLa Cells Humans Mice Mitochondria/metabolism Molecular Sequence Data Nuclear Proteins/biosynthesis,genetics,metabolism Oligodeoxyribonucleotides Phylogeny Protein Biosynthesis Recombinant Fusion Proteins/biosynthesis,metabolism Ribosomal Proteins/biosynthesis,genetics,metabolism Ribosomes Sequence Homology, Amino Acid Spinacia oleracea

Chemicals

Bacterial Proteins Cell Cycle Proteins DNA, Complementary MRPL12 protein, human Nuclear Proteins Oligodeoxyribonucleotides Recombinant Fusion Proteins Ribosomal Proteins ribosomal protein L7-L12 Glutathione Transferase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Marty L

Institut de Génétique Moléculaire, UMR5535, CNRS-Universités Montpellier I et II, France.

Fort P

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

DOI

10.1074/jbc.271.19.11468

Published

1996-05-10

Pages

11468-76

Language

English

Region

United States

NLM ID

2985121R

Subset

Databases

GENBANK

X79864, X79865

External Links

PubMed source DOI: 10.1074/jbc.271.19.11468 View journal details

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A delayed-early response nuclear gene encoding MRPL12, the mitochondrial homologue to the bacterial translational regulator L7/L12 protein.

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