Home LiteratureArticle Details
PMID: 8625413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains.

Cell ·Vol. 84 ·No. 5 ·1996-03-08 ·Pages 757-67

Brenman JE, Chao DS, Gee SH, McGee AW, Craven SE, Santillano DR, Wu Z, Huang F, Xia H, Peters MF, Froehner SC, Bredt DS

Abstract

Neuronal nitric oxide synthase (nNOS) is concentrated at synaptic junctions in brain and motor endplates in skeletal muscle. Here, we show that the N-terminus of nNOS, which contains a PDZ protein motif, interacts with similar motifs in postsynaptic density-95 protein (PSD-95) and a related novel protein, PSD-93.nNOS and PSD-95 are coexpressed in numerous neuronal populations, and a PSD-95/nNOS complex occurs in cerebellum. PDZ domain interactions also mediate binding of nNOS to skeletal muscle syntrophin, a dystrophin-associated protein. nNOS isoforms lacking a PDZ domain, identified in nNOSdelta/delta mutant mice, do not associate with PSD-95 in brain or with skeletal muscle sarcolemma. Interaction of PDZ-containing domains therefore mediates synaptic association of nNOS and may play a more general role in formation of macromolecular signaling complexes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Brain/embryology,metabolism Calcium-Binding Proteins Cell Membrane/metabolism DNA Primers Disks Large Homolog 4 Protein Embryo, Mammalian Exons Gene Expression Guanylate Kinases In Situ Hybridization Intracellular Signaling Peptides and Proteins Membrane Proteins/chemistry,metabolism Models, Structural Molecular Sequence Data Muscle Proteins/chemistry,metabolism Muscle, Skeletal/metabolism Nerve Tissue Proteins/biosynthesis,chemistry,metabolism Neurons/metabolism Nitric Oxide Synthase/biosynthesis,chemistry,metabolism Organ Specificity Polymerase Chain Reaction Protein Conformation RNA, Messenger/biosynthesis Rats Recombinant Fusion Proteins/biosynthesis,chemistry,metabolism Signal Transduction Tumor Suppressor Proteins
Chemicals
Calcium-Binding Proteins DNA Primers Disks Large Homolog 4 Protein Dlg4 protein, rat Intracellular Signaling Peptides and Proteins Membrane Proteins Mpp2 protein, rat Muscle Proteins Nerve Tissue Proteins RNA, Messenger Recombinant Fusion Proteins Tumor Suppressor Proteins postsynaptic density proteins syntrophin alpha1 Nitric Oxide Synthase DLG2 protein, human Guanylate Kinases
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Brenman J E
Department of Physiology, School of Medicine, University of California at San Fancisco, California 94143, USA.
Chao D S
Gee S H
McGee A W
Craven S E
Santillano D R
Wu Z
Huang F
Xia H
Peters M F
Froehner S C
Bredt D S
Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
Published
1996-03-08
Pages
757-67
Language
English
Region
United States
NLM ID
0413066
Subset
IM
Databases
GENBANK
U50717
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com