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PMID: 8624410 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Microsequencing and cDNA cloning of the Calvin cycle/OPPP enzyme ribose-5-phosphate isomerase (EC 5.3.1.6) from spinach chloroplasts.

Plant molecular biology ·Vol. 30 ·No. 4 ·1996-02-00 ·Pages 795-805

Martin W, Henze K, Kellerman J, Flechner A, Schnarrenberger C

Abstract

Ribose-5-phosphate isomerase (RPI) catalyses the interconversion of ribose-5-phosphate and ribulose-5-phosphate in the reductive and oxidative pentose phosphate pathways in plants. RPI from spinach chloroplasts was purified and microsequenced. Via PCR with degenerate primers designed against microsequenced peptides, a hybridisation probe was obtained and used to isolate several cDNA clones which encode RPI. The nuclear-encoded 239 amino acid mature RPI subunit has a predicted size of 25.3 kDa and is translated as a cytosolic precursor possessing a 50 amino acid transit peptide. The processing site of the transit peptide was identified from protein sequence data. Spinach leaves possess only one type of homodimeric RPI enzyme which is localized in chloroplasts and is encoded by a single nuclear gene. Molecular characterization of RPI supports the view that a single amphibolic RPI enzyme functions in the oxidative and reductive pentose phosphate pathways of spinach plastids.

MeSH Terms
Aldose-Ketose Isomerases Amino Acid Sequence Base Sequence Blotting, Southern Carbohydrate Epimerases/chemistry,classification,genetics Chloroplasts/enzymology,genetics Cloning, Molecular DNA, Complementary/genetics Gene Dosage Genes, Plant Molecular Sequence Data Pentose Phosphate Pathway Peptide Fragments/chemistry Plant Proteins/genetics Polymerase Chain Reaction Sequence Analysis/methods Sequence Homology, Amino Acid Species Specificity Spinacia oleracea/enzymology,genetics
Chemicals
DNA, Complementary Peptide Fragments Plant Proteins Carbohydrate Epimerases Aldose-Ketose Isomerases ribosephosphate isomerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Martin W
Institut für Genetik, Technische Universität Braunschweig, Germany.
Henze K
Kellerman J
Flechner A
Schnarrenberger C
References (36)
36 references, click to expand
  1. Calvin cycle multienzyme complexes are bound to chloroplast thylakoid membranes of higher plants in situ.
    Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5514-8 PMID: 11607406
  2. Enzymatic Evidence for a Complete Oxidative Pentose Phosphate Pathway in Chloroplasts and an Incomplete Pathway in the Cytosol of Spinach Leaves.
    Plant Physiol. 1995 Jun;108(2):609-614 PMID: 12228497
  3. Chloroplast phosphoribulokinase associates with yeast phosphoriboisomerase in the presence of substrate.
    FEBS Lett. 1991 Mar 25;280(2):259-61 PMID: 1707371
  4. Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme.
    Plant Mol Biol. 1994 Dec;26(6):1961-73 PMID: 7858230
  5. The six genes of the Rubisco small subunit multigene family from Mesembryanthemum crystallinum, a facultative CAM plant.
    Mol Gen Genet. 1993 Jun;239(3):450-62 PMID: 8316216
  6. The neighbor-joining method: a new method for reconstructing phylogenetic trees.
    Mol Biol Evol. 1987 Jul;4(4):406-25 PMID: 3447015
  7. A protein sequenator.
    Eur J Biochem. 1967 Mar;1(1):80-91 PMID: 6059350
  8. Identification, expression, and deduced primary structure of transketolase and other enzymes encoded within the form II CO2 fixation operon of Rhodobacter sphaeroides.
    J Biol Chem. 1991 Oct 25;266(30):20447-52 PMID: 1939098
  9. Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach.
    Plant Mol Biol. 1993 Jan;21(2):331-40 PMID: 8425060
  10. Nucleotide sequence and functional analysis of cbbR, a positive regulator of the Calvin cycle operons of Rhodobacter sphaeroides.
    J Bacteriol. 1993 Sep;175(18):5778-84 PMID: 8376325
  11. Cloning and sequencing of cDNA encoding the mature form of phosphoribulokinase from spinach.
    Gene. 1988 Jun 15;66(1):55-63 PMID: 2843430
  12. Wheat phosphoglycerate kinase: evidence for recombination between the genes for the chloroplastic and cytosolic enzymes.
    Nucleic Acids Res. 1989 Aug 25;17(16):6569-80 PMID: 2780287
  13. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  14. Escherichia coli rpiA gene encoding ribose phosphate isomerase A.
    J Bacteriol. 1993 Sep;175(17):5628-35 PMID: 8366047
  15. Ribose-5-phosphate isomerase and ribulose-5-phosphate kinase show apparent specificity for a specific ribulose 5-phosphate species.
    FEBS Lett. 1987 Feb 9;212(1):45-8 PMID: 3026853
  16. Nucleotide distribution in gymnosperm nuclear sequences suggests a model for GC-content change in land-plant nuclear genomes.
    J Mol Evol. 1994 Jul;39(1):34-46 PMID: 8064871
  17. Analysis of the primary structure of the chloroplast isozyme of triosephosphate isomerase from rye leaves by protein and cDNA sequencing indicates a eukaryotic origin of its gene.
    Biochim Biophys Acta. 1995 Apr 4;1261(2):257-64 PMID: 7711069
  18. Higher-plant chloroplast and cytosolic 3-phosphoglycerate kinases: a case of endosymbiotic gene replacement.
    Plant Mol Biol. 1996 Jan;30(1):65-75 PMID: 8616244
  19. Chloroplast and cytoplasmic enzymes. 3. Pea leaf ribose 5-phosphate isomerases.
    Biochim Biophys Acta. 1971 Apr 14;235(1):245-9 PMID: 4326161
  20. Prokaryotic features of a nucleus-encoded enzyme. cDNA sequences for chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases from mustard (Sinapis alba).
    Eur J Biochem. 1986 Sep 1;159(2):323-31 PMID: 3530755
  21. The ribose 5-phosphate isomerase-encoding gene is located immediately downstream from that encoding murine immunoglobulin kappa.
    Gene. 1995 Apr 24;156(2):191-7 PMID: 7758956
  22. Spinach 5-phosphoribose isomerase. Purification and properties of the enzyme.
    Biochemistry. 1970 Jan 6;9(1):178-84 PMID: 4391500
  23. cDNA and gene sequences of wheat chloroplast sedoheptulose-1,7-bisphosphatase reveal homology with fructose-1,6-bisphosphatases.
    Eur J Biochem. 1992 May 1;205(3):1053-9 PMID: 1374332
  24. Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase.
    EMBO J. 1984 Aug;3(8):1671-9 PMID: 6479146
  25. Purification, characterization, and cDNA sequence of glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.).
    Plant J. 1994 Mar;5(3):353-61 PMID: 8180621
  26. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  27. Cloning of the amphibolic Calvin cycle/OPPP enzyme D-ribulose-5-phosphate 3-epimerase (EC 5.1.3.1) from spinach chloroplasts: functional and evolutionary aspects.
    Plant Mol Biol. 1995 Dec;29(6):1279-91 PMID: 8616224
  28. The genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase are expressed differentially in petunia leaves.
    Nucleic Acids Res. 1986 Apr 25;14(8):3325-42 PMID: 3010233
  29. A functional five-enzyme complex of chloroplasts involved in the Calvin cycle.
    Eur J Biochem. 1988 Apr 15;173(2):437-43 PMID: 2834208
  30. Nucleotide sequence, transcriptional analysis, and expression of genes encoded within the form I CO2 fixation operon of Rhodobacter sphaeroides.
    J Biol Chem. 1991 Aug 5;266(22):14646-53 PMID: 1907281
  31. [Studies on the intracellular distribution of enzymes and substrates in leaf cells. II. Localization of enzymes of the reductive and oxidative pentosephosphate cycle in chloroplasts and permeability of the chloroplast membrane to metabolites].
    Z Naturforsch B. 1967 Nov;22(11):1200-15 PMID: 4384906
  32. Evolution of the glutamine synthetase gene, one of the oldest existing and functioning genes.
    Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):3009-13 PMID: 8096645
  33. rbcS genes in Solanum tuberosum: conservation of transit peptide and exon shuffling during evolution.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):846-50 PMID: 3422467
  34. The transketolase gene family of the resurrection plant Craterostigma plantagineum: differential expression during the rehydration phase.
    EMBO J. 1995 Feb 1;14(3):610-8 PMID: 7859749
  35. The association of ribulose-1,5-bisphosphate carboxylase with phosphoriboisomerase and phosphoribulokinase.
    Biochem Biophys Res Commun. 1986 Sep 30;139(3):947-54 PMID: 3021158
  36. A nuclear gene of eubacterial origin in Euglena gracilis reflects cryptic endosymbioses during protist evolution.
    Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9122-6 PMID: 7568085
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00019012
Published
1996-02-00
Pages
795-805
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
L43068
External Links
PubMed source DOI: 10.1007/BF00019012 View journal details
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