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PMID: 8618919 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Premature translation of protamine 1 mRNA causes precocious nuclear condensation and arrests spermatid differentiation in mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 26 ·1995-12-19 ·Pages 12451-5

Lee K, Haugen HS, Clegg CH, Braun RE

Abstract

Translational control is a major form of regulating gene expression during gametogenesis and early development in many organisms. We sought to determine whether the translational repression of the protamine 1 (Prm1) mRNA is necessary for normal spermatid differentiation in mice. To accomplish this we generated transgenic animals that carry a Prm1 transgene lacking its normal 3' untranslated region. Premature translation of Prm1 mRNA caused precocious condensation of spermatid nuclear DNA, abnormal head morphogenesis, and incomplete processing of Prm2 protein. Premature accumulation of Prm1 within syncytial spermatids in mice hemizygous for the transgene caused dominant male sterility, which in some cases was accompanied by a complete arrest in spermatid differentiation. These results demonstrate that correct temporal synthesis of Prm1 is necessary for the transition from nucleohistones to nucleoprotamines.

MeSH Terms
Animals Cell Differentiation Cell Nucleus/physiology,ultrastructure Fertility Gene Expression Regulation Infertility, Male Male Mice Mice, Transgenic Morphogenesis Mosaicism Nuclear Proteins/isolation & purification,metabolism Protamines/genetics,metabolism Protein Biosynthesis RNA, Messenger/metabolism Spermatids/cytology,metabolism,physiology Testis/cytology,pathology,physiology
Chemicals
Nuclear Proteins Prm1 protein, mouse Protamines RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee K
University of Washington, Department of Genetics, Seattle 98195-7360, USA.
Haugen H S
Clegg C H
Braun R E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.26.12451
Published
1995-12-19
Pages
12451-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40375
Subset
IM
Grants
NICHD NIH HHS · HD27215 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.26.12451 PMC Full Text View journal details
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