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PMID: 8615817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effects of tyrosine nitration on the structure and function of hen egg-white lysozyme.

The Biochemical journal ·Vol. 315 ( Pt 2) ·1996-04-15 ·Pages 473-9

Richards PG, Walton DJ, Heptinstall J

Abstract

We have investigated the effects of tyrosine nitration (to form the weak acid, 3-nitrotyrosine) at positions 23 or 20 plus 23, on the structure and function of hen egg-white lysozyme. Enzyme activity against Micrococcus luteus cell-wall fragments or soluble substrates exhibits two phenomena. (a) A decrease in Km and kcat for the hydrolysis of soluble oligo- and poly-saccharides, resulting in only minor changes in the catalytic efficiency (kcat/Km) upon nitration. (b) The hydrolysis of M. luteus cell-wall fragments appeared to be dominated by electrostatic interactions with the protein, giving a decrease in enzyme activity as the 3-nitrotyrosyl group became ionized. Removal of the cell-wall anionic polymer, teichuronic acid, from M. luteus abolished this effect. The 3-nitrotyrosine group was also found to act as a fluorescence quencher of exposed tryptophan residues in lysozyme.

MeSH Terms
Animals Cell Wall/chemistry Chickens Electrochemistry Female Hydrogen-Ion Concentration Hydrolysis Kinetics Micrococcus luteus/chemistry Models, Molecular Molecular Structure Muramidase/chemistry,genetics,metabolism Nitrates/chemistry Ovum/enzymology Point Mutation Protein Conformation Substrate Specificity Tyrosine/chemistry
Chemicals
Nitrates Tyrosine Muramidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Richards P G
School of Natural and Environmental Sciences, Coventry University, U.K.
Walton D J
Heptinstall J
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24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3150473
Published
1996-04-15
Pages
473-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1217220
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3150473 PMC Full Text View journal details
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