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PMID: 8602259 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Crystal structure of a mammalian phosphoinositide-specific phospholipase C delta.

Nature ·Vol. 380 ·No. 6575 ·1996-04-18 ·Pages 595-602

Essen LO, Perisic O, Cheung R, Katan M, Williams RL

Abstract

Mammalian phosphoinositide-specific phospholipase C enzymes (PI-PLC) act as signal transducers that generate two second messengers, inositol-1,4,5-trisphosphate and diacylglycerol. The 2.4-A structure of phospholipase C delta 1 reveals a multidomain protein incorporating modules shared by many signalling proteins. The structure suggests a mechanism for membrane attachment and Ca2+-dependent hydrolysis of second-messenger precursors. The regulation and reversible membrane association of PI-PLC may serve as a model for understanding other multidomain enzymes involved in phospholipid signalling.

MeSH Terms

Amino Acid Sequence Animals Binding Sites Catalysis Cell Membrane/metabolism Crystallography, X-Ray Humans Hydrolysis Inositol 1,4,5-Trisphosphate/metabolism Isoenzymes/chemistry,metabolism Models, Molecular Molecular Conformation Molecular Sequence Data Phosphoinositide Phospholipase C Phospholipase C delta Phosphoric Diester Hydrolases/chemistry,metabolism Protein Binding Protein Conformation Rats Recombinant Proteins/chemistry,metabolism Second Messenger Systems Sequence Homology, Amino Acid

Chemicals

Isoenzymes Recombinant Proteins Inositol 1,4,5-Trisphosphate Phosphoric Diester Hydrolases Phosphoinositide Phospholipase C Phospholipase C delta

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Essen L O

Center for Protein Engineering, MRC Centre, Cambridge, UK.

Perisic O

Cheung R

Katan M

Williams R L

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/380595a0

Published

1996-04-18

Pages

595-602

Language

English

Region

England

NLM ID

0410462

Subset

Grants

Medical Research Council · MC_U105184308 · United Kingdom

Corrections

CommentIn

PMID: 8602254

External Links

PubMed source DOI: 10.1038/380595a0 View journal details

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Crystal structure of a mammalian phosphoinositide-specific phospholipase C delta.

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