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PMID: 8601475 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of the Ascobolus immersus spore color gene b2 and study in single cells of gene silencing by methylation induced premeiotically.

Genetics ·Vol. 141 ·No. 4 ·1995-12-00 ·Pages 1299-314

Colot V, Rossignol JL

Abstract

The ascomycete Ascobolus immersus has been extensively used as a model system for the genetic study of meiotic recombination. More recently, an epigenetic process, known as methylation induced premeiotically (MIP), that acts on duplicated sequences has been discovered in A. immersus and has raised a new interest in this fungus. To try and extend these studies, we have now cloned the A. immersus spore color gene b2, a well characterized recombination hot-spot. Isolation of the whole gene was verified by physical mapping of four large b2 alterations, followed by transformation and mutant rescue of a null b2 allele. Transformation was also used to duplicate b2 and subject it to MIP. As a result, we were able for the first time to observe gene silencing as early as just after meiosis and in single cells. Furthermore, we have found evidence for a modulating effect of MIP on b2 expression, depending on the region of the gene that is duplicated and hence subjected to MIP.

MeSH Terms
Ascomycota/genetics Chromosome Mapping Cloning, Molecular DNA, Recombinant Meiosis/genetics Methylation Multigene Family Mutation Spores, Fungal/genetics Transformation, Genetic Transgenes
Chemicals
DNA, Recombinant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Colot V
Institut de Génétique et Microbiologie, Université Paris-sud, Orsay, France. colot@igmors.u-psud.fr
Rossignol J L
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/141.4.1299
Email
colot@igmors.u-psud.fr
Published
1995-12-00
Pages
1299-314
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206868
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/141.4.1299 PMC Full Text View journal details
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