A permease-oxidase complex involved in high-affinity iron uptake in yeast.

Science (New York, N.Y.) ·Vol. 271 ·No. 5255 ·1996-03-15 ·Pages 1552-7

Stearman R, Yuan DS, Yamaguchi-Iwai Y, Klausner RD, Dancis A

Abstract

Iron must cross biological membranes to reach essential intracellular enzymes. Two proteins in the plasma membrane of yeast--a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene--were shown to mediate high-affinity iron uptake. FET3 expression was required for FTR1 protein to be transported to the plasma membrane. FTR1 expression was required for apo-FET3 protein to be loaded with copper and thus acquire oxidase activity. FTR1 protein also played a direct role in iron transport. Mutations in a conserved sequence motif of FTR1 specifically blocked iron transport.

MeSH Terms

Amino Acid Sequence Binding Sites Biological Transport Carrier Proteins/chemistry,genetics,metabolism Cell Membrane/metabolism Ceruloplasmin Copper/metabolism,pharmacology Endoplasmic Reticulum/metabolism Ferric Compounds/metabolism Ferritins/chemistry,metabolism Ferrous Compounds/metabolism Genes, Fungal Golgi Apparatus/metabolism Iron/metabolism Membrane Transport Proteins/chemistry,genetics,metabolism Models, Biological Molecular Sequence Data Multienzyme Complexes/metabolism Mutation Open Reading Frames Oxidation-Reduction Oxidoreductases/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transformation, Genetic

Chemicals

Carrier Proteins FTR1 protein, S cerevisiae Ferric Compounds Ferrous Compounds Membrane Transport Proteins Multienzyme Complexes Saccharomyces cerevisiae Proteins Copper Ferritins Iron Oxidoreductases Ceruloplasmin FET3 protein, S cerevisiae

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Stearman R

Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Yuan D S

Yamaguchi-Iwai Y

Klausner R D

Dancis A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.271.5255.1552

Published

1996-03-15

Pages

1552-7

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

P38993, U18917, X73124, Z36076, Z67998

Corrections

CommentIn

PMID: 8599104

External Links

PubMed source DOI: 10.1126/science.271.5255.1552 View journal details

Analysis Services

A permease-oxidase complex involved in high-affinity iron uptake in yeast.

Contact

WeChat Official Account