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PMID: 858740 Published · ppublish English Journal Article

Chitinous fibrils in the lorica of the flagellate chrysophyte Poteriochromonas stipitata (syn. Ochromonas malhamensis).

The Journal of cell biology ·Vol. 73 ·No. 2 ·1977-05-00 ·Pages 311-21

Herth W, Kuppel A, Schnepf E

Abstract

Ordered microfibrils are formed on the membrane of the cytoplasmic tail of the alga Poteriochromonas after attachment to a substrate. The ultrastructure of native and extracted stalk fibrils was studied with electron microscope methods. In addition, the structural polysaccharide was characterized by hydrolyses, separation of the monomers by thin-layer chromatography, gas-liquid chromatography and amino acid analysis, and by X-ray diffraction. The alkali-resistant fibrils yielded mostly glucosamine upon extensive hydrolysis, and showed X-ray diffraction patterns similar to those of fugal chitin. It is concluded that the resistant core of the fibrils is chitinous.

MeSH Terms
Chitin/analysis Eukaryota/analysis,ultrastructure Glucosamine/analysis Membranes/analysis X-Ray Diffraction
Chemicals
Chitin Glucosamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Herth W
Kuppel A
Schnepf E
References (24)
24 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.73.2.311
Published
1977-05-00
Pages
311-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109911
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.73.2.311 PMC Full Text View journal details
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