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PMID: 8576057 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.

Journal of bacteriology ·Vol. 178 ·No. 4 ·1996-02-00 ·Pages 1197-9

Pelicic V, Reyrat JM, Gicquel B

Abstract

Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.

MeSH Terms
Bacillus subtilis/enzymology,genetics Genes, Bacterial Genetic Markers Hexosyltransferases/genetics Microbial Sensitivity Tests Mycobacterium/drug effects,genetics Mycobacterium bovis/drug effects,genetics Recombinant Proteins Selection, Genetic Sucrose/pharmacology
Chemicals
Genetic Markers Recombinant Proteins Sucrose Hexosyltransferases levansucrase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pelicic V
Unité de Génétique Mycobactérienne, Centre National de la Recherche Scientifique URA 1300, Institut Pasteur, Paris, France. vpelicic@pasteur.fr
Reyrat J M
Gicquel B
References (18)
18 references, click to expand
  1. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg.
    Mol Gen Genet. 1972;118(2):135-60 PMID: 4628133
  2. Advances in mycobacterial genetics: new promises for old diseases.
    Immunobiology. 1992 Feb;184(2-3):147-56 PMID: 1587540
  3. Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.
    J Bacteriol. 1985 Nov;164(2):918-21 PMID: 2997137
  4. An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis.
    Gene. 1987;57(2-3):239-46 PMID: 3319780
  5. Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a "mini" mycobacterium-Escherichia coli shuttle vector.
    J Bacteriol. 1990 May;172(5):2793-7 PMID: 2158981
  6. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis.
    Mol Microbiol. 1990 Nov;4(11):1911-9 PMID: 2082148
  7. Insertional mutagenesis and illegitimate recombination in mycobacteria.
    Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5433-7 PMID: 2052623
  8. Structure and functions of the cell envelope in relation to mycobacterial virulence, pathogenicity and multiple drug resistance. 7th Forum in Microbiology.
    Res Microbiol. 1991 May;142(4):419-81 PMID: 1678545
  9. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon.
    Mol Microbiol. 1991 Jun;5(6):1447-57 PMID: 1686293
  10. Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans.
    J Bacteriol. 1992 Aug;174(16):5462-5 PMID: 1644774
  11. Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli.
    Nucleic Acids Res. 1992 Aug 11;20(15):4105 PMID: 1508702
  12. Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000.
    FEMS Microbiol Lett. 1992 Nov 1;77(1-3):181-6 PMID: 1459406
  13. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria.
    Gene. 1993 May 15;127(1):15-21 PMID: 8486283
  14. Use of conditionally counterselectable suicide vectors for allelic exchange.
    Methods Enzymol. 1994;235:458-65 PMID: 8057916
  15. Oxygen as a key developmental regulator of Rhizobium meliloti N2-fixation gene expression within the alfalfa root nodule.
    Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3759-63 PMID: 7731979
  16. The urease locus of Mycobacterium tuberculosis and its utilization for the demonstration of allelic exchange in Mycobacterium bovis bacillus Calmette-Guérin.
    Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8768-72 PMID: 7568014
  17. rpsL+: a dominant selectable marker for gene replacement in mycobacteria.
    Mol Microbiol. 1995 Jun;16(5):991-1000 PMID: 7476195
  18. [Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg].
    Mol Gen Genet. 1983;191(1):138-44 PMID: 6412036
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.4.1197-1199.1996
Email
vpelicic@pasteur.fr
Published
1996-02-00
Pages
1197-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177784
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.4.1197-1199.1996 PMC Full Text View journal details
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