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PMID: 85724 Published · ppublish English Journal Article

Rosettes from Friend leukemia virus envelope: preparation and physicochemical and partial biological characterization.

Journal of virology ·Vol. 29 ·No. 2 ·1979-02-00 ·Pages 624-32

Schneider J, Schwarz H, Hunsmann G

Abstract

Rosette-shaped particles mainly containing gp85 were isolated from Friend leukemia virus. The isolation procedure comprised lysis of the virion by Triton X-100, affinity chromatography on concanavalin A-Sepharose, and velocity sedimentation. The rosettes displayed a mean sedimentation constant of 32S and a buoyant density of 1.21 g/ml. They contained 1% Triton X-100 and about 2% phospholipid. gp85 was identified by polyacrylamide electrophoresis, staining with periodic acid-Schiff reagent, and immunoprecipitation with antisera against Friend leukemia virus gp71 and p15(E). Rosettes completely blocked the cytotoxicity of the gp71 antiserum. The ability to hemagglutinate was inhibited by antibodies to gp71.

MeSH Terms
Chromatography, Affinity Epitopes Friend murine leukemia virus/analysis,drug effects,immunology Glycoproteins/analysis,immunology Molecular Weight Phospholipids/analysis Polyethylene Glycols/pharmacology Viral Proteins/analysis,immunology
Chemicals
Epitopes Glycoproteins Phospholipids Viral Proteins Polyethylene Glycols
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schneider J
Schwarz H
Hunsmann G
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.2.624-632.1979
Published
1979-02-00
Pages
624-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353195
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.2.624-632.1979 PMC Full Text View journal details
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