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PMID: 856871 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Influence of chronic renal failure on protein synthesis and albumin metabolism in rat liver.

The Journal of clinical investigation ·Vol. 59 ·No. 5 ·1977-05-00 ·Pages 869-78

Grossman SB, Yap SH, Shafritz DA

Abstract

Chronic renal failure in rats leads to changes in hepatic protein synthesis and albumin metabolism at both the cellular and molecular level. In rats with chronic uremia (blood urea nitrogen greater than 45 mg/100 ml 1 mo after surgical reduction in renal mass), cell-free protein synthesis is reduced 30--40% in liver membrane-bound polyribosomes. Albumin synthesis by membrane-bound polysomes in uremia is reduced even more than the reduction in total protein synthesis. Activity of free polysomes remains norma. There is also intracellular accumulation of albumin in liver of uremic rats and a concomitant decrease in serum albumin. In normal liver, most intracellular albumin is located in the microsomal fraction, whereas in liver from uremic animals the excess albumin is found in the free cytosol fraction. These results can be explained either by a defect in synthesis of albumin by membrane-bound polysomes with release of newly synthesized albumin into the cytosol or by a reduced ability of polysomes synthesizing albumin to associate with the membrane fraction in rats with chronic renal failure.

MeSH Terms
Albumins/metabolism Animals Blood Urea Nitrogen Cell Membrane Cell-Free System/metabolism Kidney Failure, Chronic/metabolism Leucine/metabolism Liver/metabolism,ultrastructure Male Models, Biological Polyribosomes/metabolism Protein Biosynthesis Rats Serum Albumin/analysis
Chemicals
Albumins Serum Albumin Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grossman S B
Yap S H
Shafritz D A
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41 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108709
Published
1977-05-00
Pages
869-78
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC372295
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108709 PMC Full Text View journal details
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