Home LiteratureArticle Details
PMID: 8552617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 1 ·1996-01-09 ·Pages 260-4

Leonard BA, Podbielski A, Hedberg PJ, Dunny GM

Abstract

Conjugative transfer of the plasmid pCF10 by Enterococcus faecalis donor cells occurs in response to a peptide sex pheromone, cCF10, secreted by recipients. The plasmid-encoded cCF10 binding protein, PrgZ, is similar in sequence to binding proteins (OppAs) encoded by oligopeptide permease (opp) operons. Mutation of prgZ decreased the sensitivity of donor cells to pheromone, whereas inactivation of the chromosomal E. faecalis opp operon abolished response at physiological concentrations of pheromone. Affinity chromatography experiments demonstrated the interaction of the pheromone with several putative intracellular regulatory molecules, including an RNA molecule required for positive regulation of conjugation functions. These data suggest that processing of the pheromone signal involves recruitment of a chromosomal Opp system by PrgZ and that signaling occurs by direct interaction of internalized pheromone with intracellular effectors.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,physiology Base Sequence Carrier Proteins/metabolism Conjugation, Genetic DNA Primers/chemistry Enterococcus faecalis/physiology Macromolecular Substances Membrane Transport Proteins/metabolism Molecular Sequence Data Oligopeptides/chemistry,metabolism Pheromones/metabolism Plasmids Protein Binding Protein Sorting Signals/genetics RNA, Bacterial/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/physiology Ribonucleoproteins/physiology Signal Transduction
Chemicals
Bacterial Proteins Carrier Proteins DNA Primers Macromolecular Substances Membrane Transport Proteins Oligopeptides Pheromones PrgZ protein, Enterococcus faecalis Protein Sorting Signals RNA, Bacterial RNA, Messenger RNA-Binding Proteins Ribonucleoproteins iCF10 peptide precursor, Plasmid pCF10 peptide pheromone cCF10 oligopeptide permease, Bacteria
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leonard B A
Institute for Advanced Studies in Biological Process Technology, University of Minnesota Medical School, Minneapolis 55455, USA.
Podbielski A
Hedberg P J
Dunny G M
References (34)
34 references, click to expand
  1. Plasmid transfer in Streptococcus faecalis: production of multiple sex pheromones by recipients.
    Plasmid. 1979 Jul;2(3):454-65 PMID: 113798
  2. The sex pheromone system of Enterococcus faecalis. More than just a plasmid-collection mechanism?
    Eur J Biochem. 1994 Jun 1;222(2):235-46 PMID: 8020463
  3. Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system.
    J Bacteriol. 1985 Sep;163(3):1210-4 PMID: 3897188
  4. Identification of regions of the Streptococcus faecalis plasmid pCF-10 that encode antibiotic resistance and pheromone response functions.
    Plasmid. 1986 May;15(3):230-41 PMID: 3012615
  5. Peptide permeases modulate transformation in Streptococcus pneumoniae.
    Mol Microbiol. 1994 Jun;12(6):881-92 PMID: 7523829
  6. Nascent peptide regulation of translation.
    J Bacteriol. 1994 Nov;176(21):6415-7 PMID: 7961390
  7. The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10.
    J Bacteriol. 1994 Dec;176(23):7405-8 PMID: 7545961
  8. Pheromone-inducible conjugation in Enterococcus faecalis: interbacterial and host-parasite chemical communication.
    J Bacteriol. 1995 Feb;177(4):871-6 PMID: 7860595
  9. Competence for genetic transformation in Streptococcus pneumoniae: organization of a regulatory locus with homology to two lactococcin A secretion genes.
    Gene. 1995 Feb 3;153(1):25-31 PMID: 7883181
  10. Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis.
    Genes Dev. 1995 Mar 1;9(5):547-58 PMID: 7698645
  11. Genetic analysis of a region of the Enterococcus faecalis plasmid pCF10 involved in positive regulation of conjugative transfer functions.
    J Bacteriol. 1995 Apr;177(8):2107-17 PMID: 7721703
  12. Silver staining of proteins in polyacrylamide gels.
    Anal Biochem. 1981 Nov 15;118(1):197-203 PMID: 6175245
  13. Structure of cCF10, a peptide sex pheromone which induces conjugative transfer of the Streptococcus faecalis tetracycline resistance plasmid, pCF10.
    J Biol Chem. 1988 Oct 5;263(28):14574-8 PMID: 3139658
  14. Cloning and expression of genes encoding pheromone-inducible antigens of Enterococcus (Streptococcus) faecalis.
    J Bacteriol. 1988 Nov;170(11):5161-8 PMID: 2846512
  15. Regulation of the pAD1 sex pheromone response in Enterococcus faecalis: effects of host strain and traA, traB, and C region mutants on expression of an E region pheromone-inducible lacZ fusion.
    J Bacteriol. 1990 May;172(5):2633-41 PMID: 2158976
  16. The ami locus of the gram-positive bacterium Streptococcus pneumoniae is similar to binding protein-dependent transport operons of gram-negative bacteria.
    Mol Microbiol. 1990 Apr;4(4):633-44 PMID: 2352474
  17. The spo0K locus of Bacillus subtilis is homologous to the oligopeptide permease locus and is required for sporulation and competence.
    J Bacteriol. 1991 Feb;173(4):1388-98 PMID: 1899858
  18. The oligopeptide transport system of Bacillus subtilis plays a role in the initiation of sporulation.
    Mol Microbiol. 1991 Jan;5(1):173-85 PMID: 1901616
  19. Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis.
    J Bacteriol. 1991 Dec;173(23):7650-64 PMID: 1938961
  20. Role of the pheromone-inducible surface protein Asc10 in mating aggregate formation and conjugal transfer of the Enterococcus faecalis plasmid pCF10.
    J Bacteriol. 1991 Dec;173(23):7665-72 PMID: 1938962
  21. Cloning and nucleotide base sequence analysis of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis.
    Antimicrob Agents Chemother. 1991 Sep;35(9):1804-10 PMID: 1659306
  22. Two-way chemical signaling in Agrobacterium-plant interactions.
    Microbiol Rev. 1992 Mar;56(1):12-31 PMID: 1579105
  23. Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein.
    J Bacteriol. 1992 Jun;174(12):4064-9 PMID: 1597420
  24. Cis-acting, orientation-dependent, positive control system activates pheromone-inducible conjugation functions at distances greater than 10 kilobases upstream from its target in Enterococcus faecalis.
    Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9020-4 PMID: 1384040
  25. Gene regulation by phosphate in enteric bacteria.
    J Cell Biochem. 1993 Jan;51(1):47-54 PMID: 8432742
  26. Identification, characterization, and nucleotide sequence of a region of Enterococcus faecalis pheromone-responsive plasmid pAD1 capable of autonomous replication.
    J Bacteriol. 1993 Apr;175(7):1900-9 PMID: 8384618
  27. Bacterial sex pheromone-induced plasmid transfer.
    Cell. 1993 Apr 9;73(1):9-12 PMID: 8462105
  28. High-efficiency gene inactivation and replacement system for gram-positive bacteria.
    J Bacteriol. 1993 Jun;175(11):3628-35 PMID: 8501066
  29. Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria.
    Microbiol Rev. 1993 Jun;57(2):320-46 PMID: 8336670
  30. Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function.
    J Bacteriol. 1993 Aug;175(16):5253-9 PMID: 8349565
  31. Characterization of the traC determinant of the Enterococcus faecalis hemolysin-bacteriocin plasmid pAD1: binding of sex pheromone.
    J Bacteriol. 1993 Aug;175(16):5260-4 PMID: 8349566
  32. Biochemical and genetic characterization of a competence pheromone from B. subtilis.
    Cell. 1994 Apr 22;77(2):207-16 PMID: 8168130
  33. The structural basis of sequence-independent peptide binding by OppA protein.
    Science. 1994 Jun 10;264(5165):1578-81 PMID: 8202710
  34. Transcriptional analysis of a region of the Enterococcus faecalis plasmid pCF10 involved in positive regulation of conjugative transfer functions.
    J Bacteriol. 1995 Apr;177(8):2118-24 PMID: 7536732
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.1.260
Published
1996-01-09
Pages
260-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40218
Subset
IM
Grants
NIAID NIH HHS · 1F32 AI08742 · United States
NIGMS NIH HHS · 1RO1 GM49530 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.1.260 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2