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PMID: 8549798 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Enzymatic characterization of purified NS3 serine proteinase of hepatitis C virus expressed in Escherichia coli.

FEBS letters ·Vol. 378 ·No. 1 ·1996-01-02 ·Pages 37-42

Mori A, Yamada K, Kimura J, Koide T, Yuasa S, Yamada E, Miyamura T

Abstract

Non-structural protein 3 (NS3) of the hepatitis C virus (HCV) has been shown to be a serine proteinase which cleaves the HCV polyprotein thus activating its replicative machinery. To characterize enzymatic activities of NS3 serine proteinase, the proteinase region was expressed in Escherichia coli and purified. The purified proteinase specifically cleaved a purified fusion protein sandwiching the NS5A/5B cleavage sequence. In addition to serine proteinase inhibitors, some chelators also inhibited the cleavage activity. Metal ions were not required for its activity, suggesting that the proteinase may be a novel serine proteinase having a unique binding site for chelators.

MeSH Terms

ATP-Binding Cassette Transporters Amino Acid Sequence Binding Sites Blotting, Western Carrier Proteins/chemistry,genetics,metabolism Chelating Agents/pharmacology DNA, Viral/genetics Escherichia coli/genetics Escherichia coli Proteins Gene Expression Hepacivirus/enzymology,genetics Maltose-Binding Proteins Molecular Sequence Data Monosaccharide Transport Proteins Protease Inhibitors/pharmacology Recombinant Fusion Proteins/metabolism Recombinant Proteins/metabolism Staphylococcal Protein A/chemistry,genetics,metabolism Substrate Specificity Viral Nonstructural Proteins/chemistry,genetics,metabolism

Chemicals

ATP-Binding Cassette Transporters Carrier Proteins Chelating Agents DNA, Viral Escherichia coli Proteins Maltose-Binding Proteins Monosaccharide Transport Proteins NS3 protein, hepatitis C virus Protease Inhibitors Recombinant Fusion Proteins Recombinant Proteins Staphylococcal Protein A Viral Nonstructural Proteins maltose transport system, E coli

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Mori A

Laboratory of Molecular Medicine, Yokohama Research Center, Mitsubishi Chemical Corp., Japan.

Yamada K

Kimura J

Koide T

Yuasa S

Yamada E

Miyamura T

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(95)01423-3

Published

1996-01-02

Pages

37-42

Language

English

Region

England

NLM ID

0155157

Subset

Databases

GENBANK

AB013620, AB013621, AB013622, AB013623, AB013624, AB013625, AB013626, AB013627, AB013628, AB013629, AB013630, AB013631, AB013632, AB013633, AB013634, AB013635, AB013636, AB013637, AB013638, AB013639, AB013640, AB013641, AB013642, AB013643, AB013644, AB013645, AB013646

External Links

PubMed source DOI: 10.1016/0014-5793(95)01423-3 View journal details

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Enzymatic characterization of purified NS3 serine proteinase of hepatitis C virus expressed in Escherichia coli.

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