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PMID: 8546709 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Annexin II up-regulates cellular levels of p11 protein by a post-translational mechanisms.

The Biochemical journal ·Vol. 313 ( Pt 1) ·1996-01-01 ·Pages 51-5

Puisieux A, Ji J, Ozturk M

Abstract

Annexin II (p36) and p11, which belong to two different families of calcium-binding proteins, are able to form a heterotetrameric protein complex (p36)2(p11)2 called calpactin I. As these proteins were detectable only in the presence of each other in a variety of cell lines, we studied the mechanisms of regulation of cellular levels of annexin II and p11. In cells expressing p11 messenger RNA, p11 protein is undetectable unless annexin II is also expressed. As an example, the hepatoblastoma HepG2 cell line displays no detectable annexin II nor p11 protein, although it expresses p11 mRNA. The overexpression of annexin II by gene transfer into HepG2 cells leads to the up-regulation of the cellular levels of p11 by a post-translational mechanism. In the presence of annexin II, there is no major change in the p11 transcript levels, but the half-life of the p11 protein is increased more than 6-fold. Thus, the degree of expression of annexin II, which varies according to different states of cellular differentiation and transformation, is an essential factor in the regulation of cellular levels of p11.

MeSH Terms
Annexin A2/metabolism Base Sequence Blotting, Northern Carcinoma, Hepatocellular/metabolism Humans Liver Neoplasms/metabolism Molecular Sequence Data Peptides/metabolism Protein Processing, Post-Translational RNA, Messenger/metabolism S100 Proteins Tumor Cells, Cultured Up-Regulation/physiology
Chemicals
Annexin A2 Peptides RNA, Messenger S100 Proteins S100 calcium binding protein A10
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Puisieux A
Laboratoire d'Oncologie Moléculaire, INSERM CJF 9302, Centre León Bérard, Lyon, France.
Ji J
Ozturk M
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3130051
Published
1996-01-01
Pages
51-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1216908
Subset
IM
Grants
NCI NIH HHS · CA-54567 · United States
External Links
PubMed source DOI: 10.1042/bj3130051 PMC Full Text View journal details
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