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PMID: 8524308 Published · ppublish English Journal Article

Yeast nucleoporin mutants are defective in pre-tRNA splicing.

Molecular and cellular biology ·Vol. 16 ·No. 1 ·1996-01-00 ·Pages 294-301

Sharma K, Fabre E, Tekotte H, Hurt EC, Tollervey D

Abstract

We have screened nucleoporin mutants for the inhibition of tRNA splicing, which has previously been proposed to be coupled to transport. Strains mutant for Nup49p or Nup116p, or genetically depleted of Nup145p, strongly accumulated unspliced pre-tRNAs. Splicing was inhibited for all 10 families of intron-containing pre-tRNA, but no effects on 5' or 3' end processing were detected. Strains mutant for Nup133p or Nsp1p accumulated lower levels of several unspliced pre-tRNAs. In contrast, no accumulation of any pre-tRNA was observed in strains mutant for Nup1p, Nup85p, or Nup100p. Other RNA processing reactions tested, pre-rRNA processing, pre-mRNA splicing, and small nucleolar and small nuclear RNA synthesis, were not clearly affected for any nucleoporin mutant. These data provide evidence for a coupling between pre-tRNA splicing and nuclear-cytoplasmic transport. Mutation of NUP49, NUP116, or NUP145 has previously been shown to lead to nuclear poly(A)+ RNA accumulation, indicating that these nucleoporins play roles in the transport of more than one class of RNA.

MeSH Terms
Base Sequence DNA, Fungal/genetics Fungal Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Nuclear Envelope/metabolism Nuclear Proteins/genetics,metabolism RNA Precursors/genetics,metabolism RNA Splicing/genetics RNA, Fungal/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism
Chemicals
DNA, Fungal Fungal Proteins Membrane Proteins Nuclear Proteins RNA Precursors RNA, Fungal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sharma K
European Molecular Biology Laboratory, Heidelberg, Germany.
Fabre E
Tekotte H
Hurt E C
Tollervey D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.1.294
Published
1996-01-00
Pages
294-301
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231003
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.1.294 PMC Full Text View journal details
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