Analysis of intrinsic and CDC25-stimulated guanine nucleotide exchange of p21ras-nucleotide complexes by fluorescence measurements.

Animals Cell Cycle Proteins/genetics,isolation & purification,metabolism Chromatography, High Pressure Liquid Escherichia coli/genetics Fluorescent Dyes/chemical synthesis,chemistry,metabolism Guanine Nucleotide Exchange Factors Guanine Nucleotides/chemical synthesis,chemistry,metabolism In Vitro Techniques Kinetics Mice Phosphoprotein Phosphatases/genetics,isolation & purification,metabolism Proteins/genetics,isolation & purification,metabolism Proto-Oncogene Proteins p21(ras)/metabolism Recombinant Fusion Proteins/genetics,isolation & purification,metabolism Spectrometry, Fluorescence ortho-Aminobenzoates/chemical synthesis,chemistry,metabolism ras Guanine Nucleotide Exchange Factors ras-GRF1

Cell Cycle Proteins Fluorescent Dyes Guanine Nucleotide Exchange Factors Guanine Nucleotides Proteins Recombinant Fusion Proteins ortho-Aminobenzoates ras Guanine Nucleotide Exchange Factors ras-GRF1 Phosphoprotein Phosphatases Proto-Oncogene Proteins p21(ras)