Home LiteratureArticle Details
PMID: 8523534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Receptor (CD46) modulation and complement-mediated lysis of uninfected cells after contact with measles virus-infected cells.

Journal of virology ·Vol. 70 ·No. 1 ·1996-01-00 ·Pages 255-63

Schneider-Schaulies J, Schnorr JJ, Schlender J, Dunster LM, Schneider-Schaulies S, ter Meulen V

Abstract

Recently, it has been observed that the infection of human target cells with certain measles virus (MV) strains leads to the downregulation of the major MV receptor CD46. Here we report that CD46 downregulation can be rapidly induced in uninfected cells after surface contact with MV particles or MV-infected cells. Receptor modulation is detectable after 30 min of cocultivation of uninfected cells with MV-infected cells and is complete after 2 to 4 h, a time after which newly synthesized MV hemagglutinin (MV-H) cannot be detected in freshly infected target cells. This contact-mediated receptor modulation is also induced by recombinant MV-H expressed by vaccinia virus and is inhibitable with antibodies against CD46 and MV-H. By titrating the effect with MV Edmonston strain-infected cells, a significant contact-mediated CD46 modulation was detectable up to a ratio of 1 infected to 64 uninfected cells. As a result of CD46 downregulation, an increased susceptibility of uninfected cells for complement-mediated lysis was observed. This phenomenon, however, is MV strain dependent, as observed for the downregulation of CD46 after MV infection. These data suggest that in acute measles or following measles vaccination, uninfected cells might also be destroyed by complement after contacting an MV-infected cell.

MeSH Terms
Animals Antigens, CD/immunology,metabolism Cell Line Cell Membrane/metabolism Chlorocebus aethiops Complement Hemolytic Activity Assay Complement System Proteins/immunology Down-Regulation Endocytosis Hemagglutinins, Viral/immunology,metabolism Humans Hydrogen-Ion Concentration Measles virus/immunology,isolation & purification,physiology Membrane Cofactor Protein Membrane Glycoproteins Receptors, Virus/metabolism Tumor Cells, Cultured Vero Cells
Chemicals
Antigens, CD CD46 protein, human Hemagglutinins, Viral Membrane Cofactor Protein Membrane Glycoproteins Receptors, Virus hemagglutinin protein G, measles virus Complement System Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schneider-Schaulies J
Institut für Virologie und Immunbiologie, Würzburg, Germany.
Schnorr J J
Schlender J
Dunster L M
Schneider-Schaulies S
ter Meulen V
References (49)
49 references, click to expand
  1. Multiple isoforms of CD46 (membrane cofactor protein) serve as receptors for measles virus.
    Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2161-5 PMID: 8134365
  2. The human CD46 molecule is a receptor for measles virus (Edmonston strain).
    Cell. 1993 Oct 22;75(2):295-305 PMID: 8402913
  3. Measles virus receptor properties are shared by several CD46 isoforms differing in extracellular regions and cytoplasmic tails.
    J Gen Virol. 1994 Sep;75 ( Pt 9):2163-71 PMID: 8077916
  4. Different membrane cofactor protein (CD46) isoforms protect transfected cells against antibody and complement mediated lysis.
    Transpl Immunol. 1993;1(2):101-8 PMID: 8081768
  5. Binding of measles virus to membrane cofactor protein (CD46): importance of disulfide bonds and N-glycans for the receptor function.
    J Virol. 1994 Oct;68(10):6299-304 PMID: 8083969
  6. Vpu-mediated proteolysis of gp160/CD4 chimeric envelope glycoproteins in the endoplasmic reticulum: requirement of both the anchor and cytoplasmic domains of CD4.
    Virology. 1994 Oct;204(1):357-66 PMID: 8091666
  7. Antigenic analysis of current wild type and vaccine strains of measles virus.
    J Infect Dis. 1994 Oct;170(4):795-801 PMID: 7930720
  8. Glycosyl-phosphatidylinositol-anchored and transmembrane forms of CD46 display similar measles virus receptor properties: virus binding, fusion, and replication; down-regulation by hemagglutinin; and virus uptake and endocytosis for antigen presentation by major histocompatibility complex class II molecules.
    J Virol. 1994 Dec;68(12):7891-9 PMID: 7966579
  9. Modulation of complement regulatory function and measles virus receptor function by the serine-threonine-rich domains of membrane cofactor protein (CD46).
    Biochem J. 1994 Nov 15;304 ( Pt 1):169-75 PMID: 7998929
  10. Physical association of moesin and CD46 as a receptor complex for measles virus.
    J Virol. 1995 Apr;69(4):2248-56 PMID: 7884872
  11. Measles virus and C3 binding sites are distinct on membrane cofactor protein (CD46).
    Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2303-7 PMID: 7534417
  12. Temporal and geographical distribution of measles virus genotypes.
    J Gen Virol. 1995 May;76 ( Pt 5):1173-80 PMID: 7730801
  13. Receptor usage and differential downregulation of CD46 by measles virus wild-type and vaccine strains.
    Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3943-7 PMID: 7732009
  14. Measles virus-induced down-regulation of CD46 is associated with enhanced sensitivity to complement-mediated lysis of infected cells.
    Eur J Immunol. 1995 Apr;25(4):976-84 PMID: 7737301
  15. Apoptosis as a cause of death in measles virus-infected cells.
    J Virol. 1995 Jun;69(6):3955-8 PMID: 7745753
  16. Detection of measles virus genome directly from clinical samples by reverse transcriptase-polymerase chain reaction and genetic variability.
    Virus Res. 1995 Jan;35(1):1-16 PMID: 7754670
  17. Immune responses during measles virus infection.
    Curr Top Microbiol Immunol. 1995;191:117-34 PMID: 7789155
  18. Measles virus-mononuclear cell interactions.
    Curr Top Microbiol Immunol. 1995;191:85-100 PMID: 7789164
  19. Moesin, and not the murine functional homologue (Crry/p65) of human membrane cofactor protein (CD46), is involved in the entry of measles virus (strain Edmonston) into susceptible murine cell lines.
    J Gen Virol. 1995 Aug;76 ( Pt 8):2085-9 PMID: 7636492
  20. Differential downregulation of CD46 by measles virus strains.
    J Virol. 1995 Nov;69(11):7257-9 PMID: 7474148
  21. Measles virus: both the haemagglutinin and fusion glycoproteins are required for fusion.
    J Gen Virol. 1991 Feb;72 ( Pt 2):439-42 PMID: 1993882
  22. Distribution of measles antigen and immunoglobulin-containing cells in the CNS in subacute sclerosing panencephalitis (SSPE) and atypical measles encephalitis.
    J Neurol Sci. 1982 Jan;53(1):29-43 PMID: 7035621
  23. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):763-7 PMID: 6096719
  24. T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):767-8 PMID: 6083454
  25. Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV.
    Science. 1986 Nov 28;234(4780):1123-7 PMID: 3095925
  26. Fatal measles infection in marmosets pathogenesis and prophylaxis.
    Infect Immun. 1980 Mar;27(3):969-78 PMID: 6769812
  27. Phorbol ester-induced downregulation of CD4 is a multistep process involving dissociation from p56lck, increased association with clathrin-coated pits, and altered endosomal sorting.
    J Exp Med. 1993 Oct 1;178(4):1209-22 PMID: 8376930
  28. Moesin: a cell membrane protein linked with susceptibility to measles virus infection.
    Virology. 1994 Jan;198(1):265-74 PMID: 8259662
  29. Nef induces CD4 endocytosis: requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain.
    Cell. 1994 Mar 11;76(5):853-64 PMID: 8124721
  30. Viruses perturb lymphocyte functions: selected principles characterizing virus-induced immunosuppression.
    Annu Rev Immunol. 1987;5:279-304 PMID: 3036182
  31. HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene product.
    Nature. 1987 Nov 19-25;330(6145):266-9 PMID: 3118220
  32. Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells: mechanism for persistence in AIDS.
    Cell. 1988 May 6;53(3):483-96 PMID: 2966682
  33. Acute measles in patients with and without neurological involvement: distribution of measles virus antigen and RNA.
    J Infect Dis. 1988 Aug;158(2):433-42 PMID: 3042879
  34. Measles virus-induced suppression of lymphocyte proliferation.
    Cell Immunol. 1988 Oct 15;116(2):367-81 PMID: 2460251
  35. Cell surface comodulation of CD4 and T cell receptor by anti-CD4 monoclonal antibody.
    J Immunol. 1989 Jul 15;143(2):397-402 PMID: 2786906
  36. The activation of protein kinase C is not necessary for the monoclonal antibody-induced modulation of CD3 and CD4 antigens.
    Eur J Immunol. 1990 May;20(5):1197-200 PMID: 2141572
  37. Intracellular interaction of human immunodeficiency virus type 1 (ARV-2) envelope glycoprotein gp160 with CD4 blocks the movement and maturation of CD4 to the plasma membrane.
    J Virol. 1990 Dec;64(12):6297-304 PMID: 2243395
  38. Expression of measles virus RNA in peripheral blood mononuclear cells of patients with measles, SSPE, and autoimmune diseases.
    Virology. 1991 Jun;182(2):703-11 PMID: 2024495
  39. Membrane cofactor protein (MCP or CD46): newest member of the regulators of complement activation gene cluster.
    Annu Rev Immunol. 1991;9:431-55 PMID: 1910685
  40. Cerebral endothelial cell infection by measles virus in subacute sclerosing panencephalitis: ultrastructural and in situ hybridization evidence.
    Neuropathol Appl Neurobiol. 1991 Aug;17(4):289-97 PMID: 1944804
  41. Measles virus inhibits mitogen-induced T cell proliferation but does not directly perturb the T cell activation process inside the cell.
    Virology. 1992 Mar;187(1):280-9 PMID: 1736530
  42. Construction of vaccinia virus recombinants expressing several measles virus proteins and analysis of their efficacy in vaccination of mice.
    J Gen Virol. 1992 Feb;73 ( Pt 2):359-67 PMID: 1538193
  43. Measles virus haemagglutinin induces down-regulation of gp57/67, a molecule involved in virus binding.
    J Gen Virol. 1993 Jun;74 ( Pt 6):1073-9 PMID: 8509760
  44. Changes in cytokine production after measles virus vaccination: predominant production of IL-4 suggests induction of a Th2 response.
    Clin Immunol Immunopathol. 1993 May;67(2):171-7 PMID: 8519092
  45. Differential induction of cytokines by primary and persistent measles virus infections in human glial cells.
    Virology. 1993 Jul;195(1):219-28 PMID: 7686310
  46. MxA-dependent inhibition of measles virus glycoprotein synthesis in a stably transfected human monocytic cell line.
    J Virol. 1993 Aug;67(8):4760-8 PMID: 8392613
  47. Human immunodeficiency virus type 1 Vpu protein induces degradation of chimeric envelope glycoproteins bearing the cytoplasmic and anchor domains of CD4: role of the cytoplasmic domain in Vpu-induced degradation in the endoplasmic reticulum.
    J Virol. 1993 Sep;67(9):5538-49 PMID: 8350411
  48. Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus.
    J Virol. 1993 Oct;67(10):6025-32 PMID: 8371352
  49. CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus.
    Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3872-6 PMID: 7909607
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.1.255-263.1996
Published
1996-01-00
Pages
255-63
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189812
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.1.255-263.1996 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2