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PMID: 8508769 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Aspergillus nidulans brlA regulatory locus consists of overlapping transcription units that are individually required for conidiophore development.

The EMBO journal ·Vol. 12 ·No. 6 ·1993-06-00 ·Pages 2439-47

Prade RA, Timberlake WE

Abstract

The Aspergillus nidulans brlA locus controls conidiophore development in conjunction with the products of several other regulatory loci. In this paper, we show that the brlA locus consists of overlapping transcription units, designated alpha and beta, with alpha transcription initiating within beta intronic sequences. The predicted BrlA polypeptides differ by 23 amino acid residues at their N-termini. Targeted mutations specifically eliminating either the alpha or beta transcript led to developmental abnormalities similar to those produced by previously identified hypomorphic mutants, showing that both transcripts have essential functions for normal development. However, provision of additional doses of alpha in a beta- strain or of beta in an alpha- strain remediated the developmental defects, indicating that the polypeptides have redundant functions. It is likely that differential regulation of alpha and beta expression in the wild type is important for the initiation and temporal regulation of development.

Related Genes
brlA
MeSH Terms
Amino Acid Sequence Aspergillus nidulans/genetics,physiology Base Sequence DNA, Fungal Fungal Proteins/genetics Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Mutation RNA, Fungal/metabolism Spores, Fungal Transcription Factors Transcription, Genetic Zinc Fingers
Chemicals
BRLA protein, Emericella nidulans DNA, Fungal Fungal Proteins RNA, Fungal Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prade R A
Department of Genetics, University of Georgia, Athens 30602.
Timberlake W E
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-06-00
Pages
2439-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413479
Subset
IM
Grants
NIGMS NIH HHS · GM37886 · United States
External Links
PubMed source PMC Full Text View journal details
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