Abstract
The effects of intravascular application of endotoxin-depleted Escherichia coli hemolysin (HlyA) was studied in rabbits and monkeys. In rabbits, bolus application of HlyA calculated to effect final blood levels of approximately 2-3 HU/ml (200-300 ng/ml) caused an acute fall of polymorphonuclear blood leukocytes to less than 20% of starting levels within 5 min. Additionally, platelet counts dropped to approximately 30% of starting levels, whereas lymphocyte counts varied considerably and seldom fell to less than 50%. Nine out ten animals that received 2-4 HU/ml toxin died within 90 min post application. These animals presented with signs of acute respiratory failure and post mortem inspection of the internal organs revealed hemorrhagic pulmonary edema. Other internal organs appeared unaffected. Application of less than 1 HU/ml HlyA was never fatal (n = 9), and only transient leukopenia was noted. Monkeys presented with a remarkable and different response. Two animals were repeatedly given HlyA at high doses ranging from 3 to 10 HU/ml. Both animals developed selective granulocytopenia, but following a short, transient drop in blood pressure they showed no severe clinical signs of cardiovascular or pulmonary malfunction. Histological examinations revealed accumulation of polymorphonuclear granulocytes in both animals in liver, lung and spleen. Very high leukocyte elastase levels were measured in one animal over a period of 1.5 h. The present results demonstrate a remarkable tolerance of monkeys towards the leukocidal effects of E. coli hemolysin. Lethality in rabbits must be due to additional effects of the toxin, possibly on cells in the pulmonary vasculature. Neither pulmonary sequestration of granulocytes nor massive release of elastase from these cells is in itself sufficient to provoke pulmonary dysfunction in monkeys.
MeSH Terms
Agranulocytosis/chemically induced
Animals
Bacterial Proteins/administration & dosage,toxicity
Bacterial Toxins/administration & dosage,toxicity
Blood Cell Count/drug effects
Blood Platelets/drug effects
Blood Pressure/drug effects
Capillaries/drug effects
Dose-Response Relationship, Drug
Escherichia coli
Escherichia coli Proteins
Granulocytes/drug effects
Hemolysin Proteins/administration & dosage,toxicity
Injections, Intra-Arterial
Injections, Intravenous
Lethal Dose 50
Liver/drug effects,pathology
Lung/blood supply,drug effects,pathology
Macaca fascicularis
Pancreatic Elastase/blood
Pulmonary Edema/chemically induced
Rabbits
Spleen/drug effects,pathology
Chemicals
Bacterial Proteins
Bacterial Toxins
Escherichia coli Proteins
Hemolysin Proteins
Hlya protein, E coli
Pancreatic Elastase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vagts D
Institute of Medical Microbiology, University of Mainz, Germany.
Dienes H P
Barth P J
Ronneberger H
Hungerer K D
Bhakdi S
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