Home LiteratureArticle Details
PMID: 849657 Published · ppublish English Journal Article

Fixation of human anti-actin autoantibodies on skeletal muscle fibres.

Clinical and experimental immunology ·Vol. 27 ·No. 2 ·1977-02-00 ·Pages 278-84

Chaponnier C, Kohler L, Gabbiani G

Abstract

Sera from five patients with chronic aggressive hepatitis containing smooth muscle autoantibodies were tested by means of indirect immunofluorescence for their binding to isolated rabbit skeletal muscle myofibrils. In all cases, the immunofluorescent staining was sharply localized to I bands. After incubation of these sera with skeletal muscle troponin-torpomyosin complex, purified troponin or purified tropomyosin, no changes in immunofluorescent staining of myofibrils were noted. However, the staining was abolished after incubation of the sera with skeletal muscle actin. In double immunodiffusion experiments, a single precipitation line was obtained after diffusion of the sera against crude or purified actin. It is concluded that, at least for the sera examined, smooth muscle autoantibodies are anti-actin autoantibodies. The high titre of such autoantibodies and their availability in clinical immunology laboratories make them a useful tool to study actin distribution in muscular and non-muscular cells.

MeSH Terms
Actins/immunology Autoantibodies Binding Sites, Antibody Hepatitis/immunology Humans Muscles/immunology
Chemicals
Actins Autoantibodies
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chaponnier C
Kohler L
Gabbiani G
References (23)
23 references, click to expand
  1. The nature of the extra protein fraction from myofibrils of striated muscle.
    Biochem J. 1959 Feb;71(2):220-8 PMID: 13628558
  2. A study of the effects of substrate concentration and certain relaxing factors on the magnesium-activated myofibrillar adenosine triphosphatase.
    Biochem J. 1956 Sep;64(1):184-92 PMID: 13363825
  3. Human smooth muscle autoantibody. Its identification as antiactin antibody and a study of its binding to "nonmuscular" cells.
    Am J Pathol. 1973 Sep;72(3):473-88 PMID: 4125700
  4. Actin and myosin and cell movement.
    CRC Crit Rev Biochem. 1974 Jan;2(1):1-65 PMID: 4273099
  5. Myosin in cultured fibroblasts.
    J Biol Chem. 1974 Jun 25;249(12):3903-7 PMID: 4365740
  6. The preparation of tropomyosin and troponin from natural actomyosin.
    Biochim Biophys Acta. 1969 Mar;175(2):301-19 PMID: 4238075
  7. A unified theory of the control of actin and myosin in nonmuscle movements.
    Cell. 1974 Jul;2(3):123-35 PMID: 4137519
  8. Granulation tissue as a contractile organ. A study of structure and function.
    J Exp Med. 1972 Apr 1;135(4):719-34 PMID: 4336123
  9. Troponin-tropomyosin complex. Column chromatographic separation and activity of the three, active troponin components with and without tropomyosin present.
    J Biol Chem. 1974 Aug 10;249(15):4742-8 PMID: 4276966
  10. Isolation and characterization of myosin from cloned mouse fibroblasts.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3693-7 PMID: 4264772
  11. Studies on thrombosthenin a, the actin-like moiety of the contractile protein from blood platelets. I. Isolation, characterization and evidence for two forms of thrombosthenin A.
    Biochim Biophys Acta. 1972 Oct 31;278(3):577-84 PMID: 4263860
  12. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
    J Biol Chem. 1971 Aug 10;246(15):4866-71 PMID: 4254541
  13. Actin antibody: the specific visualization of actin filaments in non-muscle cells.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268-72 PMID: 4210210
  14. Immunofluorescent tracing of smooth muscle contractile protein antigens in tissues other than smooth muscle.
    Clin Exp Immunol. 1974 Jan;16(1):125-35 PMID: 4619586
  15. Pancreatic beta-cell web: its possible role in insulin secretion.
    Science. 1972 Mar 10;175(4026):1128-30 PMID: 4551150
  16. Phalloidin-induced hyperplasia of actin filaments in rat hepatocytes.
    Lab Invest. 1975 Nov;33(5):562-9 PMID: 171480
  17. Microfilaments in epidermal cancer cells.
    J Cell Biol. 1974 Feb;60(2):473-82 PMID: 4813215
  18. The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine.
    J Cell Biol. 1971 Dec;51(3):752-62 PMID: 4942774
  19. New hypothesis of insulin secretion.
    Nature. 1968 Sep 14;219(5159):1177-9 PMID: 4877537
  20. Actin in the cytoplasm of adrenocortical cells.
    Proc Soc Exp Biol Med. 1975 Jul;149(3):618-21 PMID: 1096163
  21. Anti-actin specificity of human smooth muscle antibodies in chronic active hepatitis.
    Clin Exp Immunol. 1976 May;24(2):266-72 PMID: 945140
  22. The actin content of fibroblasts.
    Biochem J. 1975 May;147(2):221-8 PMID: 1180890
  23. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types.
    J Cell Biol. 1969 Nov;43(2):312-28 PMID: 5344150
Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1977-02-00
Pages
278-84
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1540775
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2