The proteins in the Z line of insect flight muscle.

The Biochemical journal ·Vol. 161 ·No. 2 ·1977-02-01 ·Pages 399-403

Bullard B, Sainsbury GM

Abstract

Z discs were isolated from Lethocerus flight muscle by removing the contractile proteins from myofibrils with a solution of high ionic strength. The protein composition of the Z discs was analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the major proteins were alpha-actinin, actin and tropomyosin. Z lines were selectively removed from intact myofibrils by digestion with crude lipase and chymotrypsin, but not by purified lipase.

MeSH Terms

Animals Chymotrypsin Electrophoresis, Polyacrylamide Gel Hemiptera/analysis Lipase Muscle Proteins/analysis Muscles/analysis,ultrastructure Myofibrils/analysis

Chemicals

Muscle Proteins Lipase Chymotrypsin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bullard B

Sainsbury G M

References (20)

20 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1610399

Published

1977-02-01

Pages

399-403

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164517

Subset

External Links

PubMed source DOI: 10.1042/bj1610399 PMC Full Text View journal details

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The proteins in the Z line of insect flight muscle.

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