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PMID: 8490128 Published · ppublish English Journal Article

PCR-dependent amplification and sequence characterization of partial cDNAs encoding myosin-like proteins in Anemia phyllitidis (L.) Sw. and Arabidopsis thaliana (L.) Heynh.

Plant molecular biology ·Vol. 21 ·No. 6 ·1993-03-00 ·Pages 1077-83

Moepps B, Conrad S, Schraudolf H

Abstract

Partial cDNAs encoding for myosin-like proteins from Anemia phyllitidis and Arabidopsis thaliana have been isolated using PCR technology. The deduced amino acid sequences show an average similarity up to 62% with known myosin heavy chain genes. From northern blot analysis we were able to estimate that transcripts of ca. 6.1 kb size are expressed in A. phyllitidis.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/genetics Blotting, Northern Blotting, Southern Culture Techniques Humans Molecular Sequence Data Myosins/genetics,metabolism Plant Proteins/genetics Plants/genetics Polymerase Chain Reaction Sequence Homology, Amino Acid
Chemicals
Plant Proteins Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moepps B
Institut für Allgemeine Botanik, Universität Ulm, FRG.
Conrad S
Schraudolf H
References (18)
18 references, click to expand
  1. Myosin structure and function in cell motility.
    Annu Rev Cell Biol. 1987;3:379-421 PMID: 3318880
  2. An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.
    J Biol Chem. 1985 Mar 10;260(5):2605-8 PMID: 3838308
  3. Novel myosin heavy chain encoded by murine dilute coat colour locus.
    Nature. 1991 Feb 21;349(6311):709-13 PMID: 1996138
  4. Anti-tubulin antibodies locate the blepharoplast during spermatogenesis in the fern Platyzoma microphyllum R.Br.: a correlated immunofluorescence and electron-microscopic study.
    J Cell Sci. 1986 Mar;81:243-65 PMID: 3525583
  5. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  6. Complete nucleotide sequence of full length cDNA for rat alpha cardiac myosin heavy chain.
    Nucleic Acids Res. 1989 Sep 25;17(18):7527-8 PMID: 2798111
  7. Molecular cloning of protozoan myosin I heavy chain genes.
    J Cell Sci Suppl. 1991;14:37-40 PMID: 1885657
  8. Recent advances in the polymerase chain reaction.
    Science. 1991 Jun 21;252(5013):1643-51 PMID: 2047872
  9. Cellular myosin heavy chain in human leukocytes: isolation of 5' cDNA clones, characterization of the protein, chromosomal localization, and upregulation during myeloid differentiation.
    Blood. 1991 Oct 1;78(7):1826-33 PMID: 1912569
  10. Progressive sequence alignment as a prerequisite to correct phylogenetic trees.
    J Mol Evol. 1987;25(4):351-60 PMID: 3118049
  11. The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles.
    J Cell Biol. 1991 May;113(3):539-51 PMID: 2016335
  12. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  13. pEMBL: a new family of single stranded plasmids.
    Nucleic Acids Res. 1983 Mar 25;11(6):1645-55 PMID: 6300771
  14. Complete sequence of the Drosophila nonmuscle myosin heavy-chain transcript: conserved sequences in the myosin tail and differential splicing in the 5' untranslated sequence.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6316-20 PMID: 2117279
  15. Molecular genetic dissection of myosin heavy chain function.
    Cell. 1990 Feb 9;60(3):347-50 PMID: 2406017
  16. Identification of common molecular subsequences.
    J Mol Biol. 1981 Mar 25;147(1):195-7 PMID: 7265238
  17. Myosins of nonmuscle cells.
    Annu Rev Biophys Biophys Chem. 1988;17:23-45 PMID: 3293586
  18. Partial deduced sequence of the 110-kD-calmodulin complex of the avian intestinal microvillus shows that this mechanoenzyme is a member of the myosin I family.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2895-903 PMID: 2687288
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
Published
1993-03-00
Pages
1077-83
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
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