Abstract
Small fragments of DNA of known length were made with the polymerase chain reaction. These fragments had biotin molecules covalently attached at their ends. They were subsequently labeled with a chimeric protein fusion between streptavidin and two immunoglobulin G-binding domains of staphylococcal protein A. This tetrameric species was expected to bind up to four DNA molecules via their attached biotin moieties. The DNA-protein complex was deposited on mica and imaged with an atomic force microscope. The images revealed the protein chimera at the expected location at the ends of the strands of DNA as well as the expected dimers, trimers, and tetramers of DNA bound to a single protein.
MeSH Terms
Bacterial Proteins/chemistry,metabolism
Base Sequence
Cloning, Molecular
DNA/genetics,ultrastructure
Humans
Microscopy, Scanning Tunneling/methods
Molecular Sequence Data
Polymerase Chain Reaction/methods
Recombinant Fusion Proteins/chemistry,metabolism
Repetitive Sequences, Nucleic Acid
Staphylococcal Protein A/chemistry,metabolism
Streptavidin
Chemicals
Bacterial Proteins
Recombinant Fusion Proteins
Staphylococcal Protein A
DNA
Streptavidin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Murray M N
Human Genome Center, Lawrence Berkeley Laboratory, Berkeley, CA 94720.
Hansma H G
Bezanilla M
Sano T
Ogletree D F
Kolbe W
Smith C L
Cantor C R
Spengler S
Hansma P K
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