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PMID: 8471030 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for acidity of prelysosomal autophagic/endocytic vacuoles (amphisomes).

The Biochemical journal ·Vol. 291 ( Pt 1) ·1993-04-01 ·Pages 115-21

Strømhaug PE, Seglen PO

Abstract

[14C]Lactose electroinjected into isolated rat hepatocytes is normally autophagocytosed, transferred to lysosomes and degraded by lysosomal beta-galactosidase, but at high concentrations of asparagine the transfer is inhibited and lactose accumulates in prelysosomal autophagic/endocytic vacuoles (amphisomes). The accumulation can be prevented by addition of yeast beta-galactosidase, which is transferred to the lactose-containing vacuoles by endocytosis. Propylamine, a weak base capable of neutralizing acidic vacuoles, protects autophagocytosed lactose against both endogenous and exogenous beta-galactosidase, suggesting that amphisomes, like lysosomes, have an acidic internal environment.

MeSH Terms
Animals Asparagine/pharmacology Autophagy/drug effects Endocytosis Hydrogen-Ion Concentration Lactose/metabolism Liver/ultrastructure Lysosomes/metabolism Male Propylamines/pharmacology Rats Rats, Wistar Vacuoles/metabolism beta-Galactosidase/metabolism
Chemicals
Propylamines Asparagine beta-Galactosidase Lactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Strømhaug P E
Department of Tissue Culture, Norwegian Radium Hospital, Oslo.
Seglen P O
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2910115
Published
1993-04-01
Pages
115-21
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132489
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2910115 PMC Full Text View journal details
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