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PMID: 8468299 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The Rhodobacter capsulatus chlorin reductase-encoding locus, bchA, consists of three genes, bchX, bchY, and bchZ.

Journal of bacteriology ·Vol. 175 ·No. 8 ·1993-04-00 ·Pages 2407-13

Burke DH, Alberti M, Hearst JE

Abstract

The bchA locus of Rhodobacter capsulatus codes for the chlorin reductase enzyme in the bacteriochlorophyll synthesis pathway. Previous work has suggested that this locus might encompass a single gene. We have sequenced the bchA locus and found it to contain three coding segments, which we designate bchX, bchY, and bchZ. Each coding segment contains its own translational initiation sequence and follows codon utilization patterns consistent with those of previously published R. capsulatus genes. When various regions of the bchA locus and flanking sequences were subcloned into an expression vector and expressed in Escherichia coli, the three coding segments were all expressed as separate peptides. Finally, conservation of amino acid sequences between bchX and a subunit of the protochlorophyllide reductase (bchL, 34% identity) and the nitrogenase Fe protein (nifH, 30 to 37% identity) suggests structural and mechanistic commonalities among all three proteins.

Related Genes
bchA bchX bchY bchZ
MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics Base Sequence Chromosome Mapping Genes, Bacterial Molecular Sequence Data Nitrogenase/genetics Open Reading Frames Oxidoreductases Protein Biosynthesis Rhodobacter capsulatus/enzymology,genetics
Chemicals
Bacterial Proteins bacteriochlorophyll A proteins, Bacteria Oxidoreductases Nitrogenase nitrogenase reductase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Burke D H
Department of Chemistry, University of California, Berkeley.
Alberti M
Hearst J E
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29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.8.2407-2413.1993
Published
1993-04-00
Pages
2407-13
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204530
Subset
IM
Grants
NIGMS NIH HHS · GM 30786 · United States
Databases
GENBANK
Z11165
External Links
PubMed source DOI: 10.1128/jb.175.8.2407-2413.1993 PMC Full Text View journal details
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