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PMID: 8467805 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Programmed cell death by bcl-2-dependent and independent mechanisms in B lymphoma cells.

The EMBO journal ·Vol. 12 ·No. 4 ·1993-04-00 ·Pages 1555-60

Cuende E, Alés-Martínez JE, Ding L, Gónzalez-García M, Martínez C, Nunez G

Abstract

Programmed cell death (PCD) or apoptosis is a common form of cellular demise during embryogenesis, tumorigenesis and clonal selection in the immune system. The bcl-2 proto-oncogene has been recently implicated as a potential physiological regulator of the PCD pathway. Gene transfer studies have shown that overexpression of bcl-2 blocks apoptosis mediated by several stimuli in cultured cell lines and promotes the survival of B and T lymphocytes in transgenic mice. However, it remains unclear whether under normal conditions bcl-2 is responsible for controlling cell death. We have investigated the role of bcl-2 in the antimembrane IgM (mIgM)-induced apoptotic death of WEHI-231 B cell lymphoma, a model that mimics clonal deletion of immature B cells by antigen. Signalling of mIgM receptors triggered downregulation of both bcl-2 RNA and protein, and induced apoptosis in WEHI-231 B cells. This effect appeared to be specific since (i) the levels of beta 2-microglobulin and beta-actin RNA remain unchanged and (ii) signalling of the apoptosis-resistant B cell lymphoma line BAL-17 with anti-mu was not associated with downregulation of bcl-2 RNA. However, stable expression of bcl-2 by transfection did not rescue WEHI-231 B cells from apoptosis, yet WEHI-231 cells overexpressing bcl-2 were more resistant to programmed cell death induced by heat-shock.(ABSTRACT TRUNCATED AT 250 WORDS)

Related Genes
c-bcl-2
MeSH Terms
Apoptosis B-Lymphocytes/physiology Gene Expression Regulation, Neoplastic Hot Temperature Humans Immunoglobulin M/physiology In Vitro Techniques Lymphoma, B-Cell/pathology Proto-Oncogene Mas Proto-Oncogene Proteins/physiology Proto-Oncogene Proteins c-bcl-2 RNA, Messenger/genetics Receptors, Antigen, B-Cell/physiology Tumor Cells, Cultured
Chemicals
Immunoglobulin M MAS1 protein, human Proto-Oncogene Mas Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 RNA, Messenger Receptors, Antigen, B-Cell
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cuende E
Centro de Biología Molecular (CSIC), Universidad Autónoma de Madrid, Spain.
Alés-Martínez J E
Ding L
Gónzalez-García M
Martínez C
Nunez G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-04-00
Pages
1555-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413368
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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