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PMID: 8467795 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Clustered organization of homologous KRAB zinc-finger genes with enhanced expression in human T lymphoid cells.

The EMBO journal ·Vol. 12 ·No. 4 ·1993-04-00 ·Pages 1363-74

Bellefroid EJ, Marine JC, Ried T, Lecocq PJ, Rivière M, Amemiya C, Poncelet DA, Coulie PG, de Jong P, Szpirer C

Abstract

KRAB zinc-finger proteins (KRAB-ZFPs) constitute a large subfamily of ZFPs of the Krüppel C2H2 type. KRAB (Krüppel-associated box) is an evolutionarily conserved protein domain found N-terminally with respect to the finger repeats. We report here the characterization of a particular subgroup of highly related human KRAB-ZFPs. ZNF91 is one representative of this subgroup and contains 35 contiguous finger repeats at its C-terminus. Three mRNA isoforms with sequence identity to ZNF91 were isolated by the polymerase chain reaction. These encode proteins with a KRAB domain present, partially deleted or absent. Five genomic fragments were characterized, each encoding part of a gene: the ZNF91 gene or one of four distinct, related KRAB-ZFP genes. All exhibit a common exon/intron organization with the variant zinc finger repeats organized in a single exon and the KRAB domain encoded by two separate exons. This positioning of introns supports the hypothesis that the mRNA isoforms encoding polypeptides with variability in the KRAB domain could arise by alternative splicing. By in situ chromosomal mapping studies and by analysis of fragments from a human genomic yeast artificial chromosome library containing KRAB-ZFP genes, we show that these genes occur in clusters; in particular, a gene complex containing over 40 genes has been identified in chromosomal region 19p12-p13.1. These ZNF91-related genes probably arose late during evolution since no homologous genes are detected in the mouse and rat genomes. Although the transcription of members of this KRAB-ZFP gene subgroup is detectable in all human tissues, their expression is significantly higher in human T lymphoid cells.

Related Genes
ZNF100 ZNF101 ZNF102 ZNF103 ZNF104 ZNF105 ZNF106 ZNF107 ZNF108 ZNF109 ZNF110 ZNF111 ZNF112 ZNF113 ZNF43 ZNF85 ZNF90 ZNF91 ZNF92 ZNF93 ZNF94 ZNF95 ZNF96 ZNF97 ZNF98
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Chromosome Mapping Chromosomes, Human, Pair 19 Cloning, Molecular DNA/genetics DNA-Binding Proteins/genetics Gene Expression Genes Genetic Linkage Humans Kruppel-Like Transcription Factors Mice Molecular Sequence Data Multigene Family RNA, Messenger/genetics Rats Restriction Mapping Sequence Alignment T-Lymphocytes/metabolism Zinc Fingers
Chemicals
DNA-Binding Proteins Kruppel-Like Transcription Factors RNA, Messenger ZNF101 protein, human DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Bellefroid E J
Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de Liège, Sart-Tilman, Belgium.
Marine J C
Ried T
Lecocq P J
Rivière M
Amemiya C
Poncelet D A
Coulie P G
de Jong P
Szpirer C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-04-00
Pages
1363-74
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413348
Subset
IM
Grants
NHGRI NIH HHS · HG00272 · United States
Databases
GENBANK
L11672
External Links
PubMed source PMC Full Text View journal details
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