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PMID: 8464925 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two distinct gene subfamilies within the family of cysteine protease genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 7 ·1993-04-01 ·Pages 3063-7

Karrer KM, Peiffer SL, DiTomas ME

Abstract

A cDNA clone for a physiologically regulated Tetrahymena cysteine protease gene was sequenced. The nucleotide sequence predicts that the clone encodes a 336-amino acid protein composed of a 19-residue N-terminal signal sequence followed by a 107-residue propeptide and a 210-residue mature protein. Comparison of the deduced amino acid sequence of the protein with those of other cysteine proteases revealed a highly conserved interspersed amino acid motif in the propeptide region of the protein, the ERFNIN motif. The motif was present in all of the cysteine proteases in the data base with the exception of the cathepsin B-like proteins, which have shorter propeptides. Differences in the propeptides and in conserved amino acids of the mature proteins suggest that the ERFNIN proteases and the cathepsin B-like proteases constitute two distinct subfamilies within the cysteine proteases.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cathepsin B/genetics Cysteine Endopeptidases/genetics Humans Molecular Sequence Data Multigene Family Plants/enzymology,genetics Sequence Homology, Amino Acid Tetrahymena thermophila/enzymology,genetics
Chemicals
Cysteine Endopeptidases Cathepsin B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Karrer K M
Department of Biology, Marquette University, Milwaukee, WI 53233.
Peiffer S L
DiTomas M E
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.7.3063
Published
1993-04-01
Pages
3063-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46237
Subset
IM
Grants
NIGMS NIH HHS · GM39890 · United States
Databases
GENBANK
L03212
External Links
PubMed source DOI: 10.1073/pnas.90.7.3063 PMC Full Text View journal details
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