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PMID: 8460128 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Chicken neuronal acetylcholine receptor alpha 2-subunit gene exhibits neuron-specific expression in the brain and spinal cord of transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 6 ·1993-03-15 ·Pages 2237-41

Daubas P, Salmon AM, Zoli M, Geoffroy B, Devillers-Thiéry A, Bessis A, Médevielle F, Changeux JP

Abstract

Transgenic mice carrying the complete structural gene of the alpha 2 subunit of the chicken neuronal nicotinic acetylcholine receptor (nAChR) and 7 kilobase pairs (kbp) of 5' upstream and 3 kbp of 3' downstream sequences have been generated. The transgene was stably integrated in transgenic lines and transmitted to their progeny. Avian transgene expression was predominant in the central nervous system as detected by specific alpha 2-subunit cDNA amplification. Moreover, in at least two independent mouse lines, its expression appeared to be neuron-specific and reproducibly restricted to subregions in the brain and spinal cord, as revealed by in situ hybridization histochemistry. Most cranial motor nuclei were positive, and several of the alpha 2-subunit transgene-expressing structures corresponded to cholinergic areas in rodents. This study reveals that regulatory mechanisms giving rise to neuronal-specific gene expression have been conserved at least in part between birds and mammals.

MeSH Terms
Animals Brain/physiology Chickens DNA/genetics,isolation & purification Gene Expression Macromolecular Substances Mice Mice, Transgenic Neurons/physiology Organ Specificity RNA, Messenger/analysis,genetics,metabolism Receptors, Cholinergic/genetics Spinal Cord/physiology Transcription, Genetic
Chemicals
Macromolecular Substances RNA, Messenger Receptors, Cholinergic DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Daubas P
Unité Associée D1284, Centre National de la Recherche Scientifique, Neurobiologie Moléculaire, Département des Biotechnologies, Institut Pasteur, Paris, France.
Salmon A M
Zoli M
Geoffroy B
Devillers-Thiéry A
Bessis A
Médevielle F
Changeux J P
References (33)
33 references, click to expand
  1. Distribution of methionine-enkephalin immunoreactivity in the chick brain: an immunohistochemical study.
    J Comp Neurol. 1981 Jul 10;199(4):513-33 PMID: 7024329
  2. Basal ganglionic pathways to the tectum: studies in reptiles.
    J Comp Neurol. 1980 Sep 15;193(2):565-89 PMID: 7440783
  3. Nicotinic binding in rat brain: autoradiographic comparison of [3H]acetylcholine, [3H]nicotine, and [125I]-alpha-bungarotoxin.
    J Neurosci. 1985 May;5(5):1307-15 PMID: 3998824
  4. Monoclonal antibodies and polyvalent antiserum to chicken choline acetyltransferase.
    J Neurochem. 1986 Mar;46(3):968-76 PMID: 3512775
  5. A 5'-flanking region of the chicken acetylcholine receptor alpha-subunit gene confers tissue specificity and developmental control of expression in transfected cells.
    Mol Cell Biol. 1987 Feb;7(2):951-5 PMID: 3821734
  6. Acetylcholine receptor synthesis rate and levels of receptor subunit messenger RNAs in chick muscle.
    Neuroscience. 1988 Jan;24(1):175-87 PMID: 3368046
  7. Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors.
    EMBO J. 1988 Mar;7(3):595-601 PMID: 3267226
  8. Localization of nicotinic acetylcholine receptor alpha-subunit transcripts during myogenesis and motor endplate development in the chick.
    J Cell Biol. 1989 Mar;108(3):1025-37 PMID: 2921278
  9. Illegitimate transcription: transcription of any gene in any cell type.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2617-21 PMID: 2495532
  10. Immunohistochemical localization of choline acetyltransferase in the chicken mesencephalon.
    J Comp Neurol. 1989 Mar 22;281(4):641-57 PMID: 2708587
  11. Interaction of nuclear factors with the upstream region of the alpha-subunit gene of chicken muscle acetylcholine receptor: variations with muscle differentiation and denervation.
    EMBO J. 1989 Mar;8(3):687-94 PMID: 2721497
  12. Distribution of alpha 2, alpha 3, alpha 4, and beta 2 neuronal nicotinic receptor subunit mRNAs in the central nervous system: a hybridization histochemical study in the rat.
    J Comp Neurol. 1989 Jun 8;284(2):314-35 PMID: 2754038
  13. Distribution of choline acetyltransferase immunopositive structures in the rat brainstem.
    Brain Res. 1989 Aug 28;495(2):271-97 PMID: 2765931
  14. Expression and regulation of neuronal acetylcholine receptor mRNA in chick ciliary ganglia.
    Neuron. 1988 Aug;1(6):495-502 PMID: 3272177
  15. Alpha 3, alpha 5, and beta 4: three members of the rat neuronal nicotinic acetylcholine receptor-related gene family form a gene cluster.
    J Biol Chem. 1990 Mar 15;265(8):4472-82 PMID: 1689727
  16. Neural regulation of gene expression by an acetylcholine receptor promoter in muscle of transgenic mice.
    Neuron. 1989 Apr;2(4):1295-300 PMID: 2627372
  17. Expression of neuronal nicotinic acetylcholine receptor genes in the developing chick visual system.
    EMBO J. 1990 Apr;9(4):1021-6 PMID: 1691091
  18. Two adjacent MyoD1-binding sites regulate expression of the acetylcholine receptor alpha-subunit gene.
    Nature. 1990 May 24;345(6273):353-5 PMID: 2342565
  19. Distinct regional expression of nicotinic acetylcholine receptor genes in chick brain.
    Brain Res Mol Brain Res. 1990 May;7(4):305-15 PMID: 2163006
  20. Brain alpha-bungarotoxin binding protein cDNAs and MAbs reveal subtypes of this branch of the ligand-gated ion channel gene superfamily.
    Neuron. 1990 Jul;5(1):35-48 PMID: 2369519
  21. Differential expression of the neuronal acetylcholine receptor alpha 2 subunit gene during chick brain development.
    Neuron. 1990 Jul;5(1):49-60 PMID: 2369520
  22. The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: beta 4.
    Neuron. 1989 Oct;3(4):487-96 PMID: 2642007
  23. A neuronal nicotinic acetylcholine receptor subunit (alpha 7) is developmentally regulated and forms a homo-oligomeric channel blocked by alpha-BTX.
    Neuron. 1990 Dec;5(6):847-56 PMID: 1702646
  24. An acetylcholine receptor alpha-subunit promoter conferring preferential synaptic expression in muscle of transgenic mice.
    EMBO J. 1991 Mar;10(3):625-32 PMID: 1900467
  25. Pharmacological and functional diversity of neuronal nicotinic acetylcholine receptors.
    Trends Pharmacol Sci. 1991 Jan;12(1):34-40 PMID: 2006540
  26. The distribution of mRNA encoded by a new member of the neuronal nicotinic acetylcholine receptor gene family (alpha 5) in the rat central nervous system.
    Brain Res. 1990 Aug 27;526(1):45-53 PMID: 2078817
  27. Mechanisms of complex transcriptional regulation: implications for brain development.
    Neuron. 1991 Aug;7(2):183-96 PMID: 1873026
  28. Selective expression of an acetylcholine receptor-lacZ transgene in synaptic nuclei of adult muscle fibers.
    Development. 1991 Dec;113(4):1181-91 PMID: 1811935
  29. Cholinergic neurons in the rat central nervous system demonstrated by in situ hybridization of choline acetyltransferase mRNA.
    Neuroscience. 1992;47(4):807-22 PMID: 1579211
  30. Localization of mRNAs coding for CMD1, myogenin and the alpha-subunit of the acetylcholine receptor during skeletal muscle development in the chicken.
    Mech Dev. 1992 Mar;37(1-2):95-106 PMID: 1318730
  31. Spatial restriction of AChR gene expression to subsynaptic nuclei.
    Development. 1992 Mar;114(3):545-53 PMID: 1618127
  32. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.
    J Histochem Cytochem. 1970 May;18(5):315-33 PMID: 4192899
  33. Basal ganglia pathways to the tectum: the afferent and efferent connections of the lateral spiriform nucleus of pigeon.
    J Comp Neurol. 1982 Jun 10;208(1):16-36 PMID: 7119152
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.6.2237
Published
1993-03-15
Pages
2237-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46061
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.6.2237 PMC Full Text View journal details
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