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PMID: 8459204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional characterization of a signal transducing motif present in the T cell antigen receptor zeta chain.

The Journal of experimental medicine ·Vol. 177 ·No. 4 ·1993-04-01 ·Pages 1093-103

Irving BA, Chan AC, Weiss A

Abstract

A conserved sequence motif has been identified in a number of signaling subunits associated with hematopoietic cell antigen receptors. Here, we characterize signaling by a 17 amino acid motif that is triplicated in the T cell antigen receptor zeta chain. Analysis of zeta truncations and constructs containing the isolated motif demonstrates that this motif is sufficient for the induction of both proximal and distal events associated with T cell activation. Stimulation of truncations that contain either one, two, or three copies of the motif results in induction of an identical pattern of tyrosine phosphoproteins. Moreover, triplication of the NH2-terminal zeta motif results in enhanced signaling, suggesting a redundant role in signal amplification for the three motifs in zeta. Finally, we demonstrate the association of a recently identified protein tyrosine kinase ZAP-70 with this motif, and provide evidence for its involvement in zeta function.

MeSH Terms
Amino Acid Sequence CD8 Antigens/genetics,metabolism Cell Line Conserved Sequence Flow Cytometry Humans Membrane Proteins/chemistry,genetics,metabolism Molecular Sequence Data Peptide Fragments/metabolism Precipitin Tests Protein-Tyrosine Kinases/metabolism Receptors, Antigen, T-Cell/chemistry,genetics,metabolism Signal Transduction T-Lymphocytes/metabolism Transfection ZAP-70 Protein-Tyrosine Kinase
Chemicals
CD8 Antigens Membrane Proteins Peptide Fragments Receptors, Antigen, T-Cell antigen T cell receptor, zeta chain Protein-Tyrosine Kinases ZAP-70 Protein-Tyrosine Kinase ZAP70 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Irving B A
Department of Physiology, University of California, San Francisco 94143.
Chan A C
Weiss A
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44 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.177.4.1093
Published
1993-04-01
Pages
1093-103
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190991
Subset
IM
Grants
NIAMS NIH HHS · AR-20684 · United States
External Links
PubMed source DOI: 10.1084/jem.177.4.1093 PMC Full Text View journal details
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