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PMID: 8443413 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell cycle expression of two replicative DNA polymerases alpha and delta from Schizosaccharomyces pombe.

Molecular biology of the cell ·Vol. 4 ·No. 2 ·1993-02-00 ·Pages 145-57

Park H, Francesconi S, Wang TS

Abstract

We have investigated the expression of two Schizosaccharomyces pombe replicative DNA polymerases alpha and delta during the cell cycle. The pol alpha+ and pol delta+ genes encoding DNA polymerases alpha and delta were isolated from S. pombe. Both pol alpha+ and pol delta+ genes are single copy genes in haploid cells and are essential for cell viability. In contrast to Saccharomyces cerevisiae homologs, the steady-state transcripts of both S. pombe pol alpha+ and pol delta+ genes were present throughout the cell cycle. Sequence analysis of the pol alpha+ and pol delta+ genes did not reveal the Mlu I motifs in their upstream sequences that are involved in cell cycle-dependent transcription of S. cerevisiae DNA synthesis genes as well as the S. pombe cdc22+ gene at the G1/S boundary. However, five near-match Mlu I motifs were found in the upstream region of the pol alpha+ gene. S. pombe DNA polymerases alpha and delta proteins were also expressed constantly throughout the cell cycle. In addition, the enzymatic activity of the S. pombe DNA polymerase alpha measured by in vitro assay was detected at all stages of the cell cycle. Thus, these S. pombe replicative DNA polymerases, like that of S. pombe cdc17+ gene, are expressed throughout the cell cycle at the transcriptional and protein level. These results indicate that S. pombe has at least two regulatory modes for the expression of genes involved in DNA replication and DNA precursor synthesis.

Related Genes
pol&agr pol&dgr
MeSH Terms
Base Sequence Cell Cycle DNA Polymerase II/genetics,immunology,metabolism DNA Polymerase III DNA Replication DNA-Directed DNA Polymerase/genetics,immunology,metabolism Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Polymerase Chain Reaction RNA, Fungal/genetics RNA, Messenger/genetics Restriction Mapping Schizosaccharomyces/cytology,enzymology,genetics
Chemicals
Oligodeoxyribonucleotides RNA, Fungal RNA, Messenger DNA Polymerase II DNA Polymerase III DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Park H
Department of Pathology, Stanford University School of Medicine, California 94305-5324.
Francesconi S
Wang T S
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.4.2.145
Published
1993-02-00
Pages
145-57
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC300911
Subset
IM
Grants
NCI NIH HHS · CA-54415 · United States
Databases
GENBANK
L07734
External Links
PubMed source DOI: 10.1091/mbc.4.2.145 PMC Full Text View journal details
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