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PMID: 8433990 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 4 ·1993-02-15 ·Pages 1315-9

Tsaneva IR, Müller B, West SC

Abstract

The SOS-inducible ruvA and ruvB gene products of Escherichia coli are required for normal levels of genetic recombination and DNA repair. In vitro, RuvA protein interacts specifically with Holliday junctions and, together with RuvB (an ATPase), promotes their movement along DNA. This process, known as branch migration, is important for the formation of heteroduplex DNA. In this paper, we show that the RuvA and RuvB proteins promote the unwinding of partially duplex DNA. Using single-stranded circular DNA substrates with annealed fragments (52-558 nucleotides in length), we show that RuvA and RuvB promote strand displacement with a 5'-->3' polarity. The reaction is ATP-dependent and its efficiency is inversely related to the length of the duplex DNA. These results show that the ruvA and ruvB genes encode a DNA helicase that specifically recognizes Holliday junctions and promotes branch migration.

Related Genes
ruvA ruvB
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Bacteriophage phi X 174/genetics,metabolism DNA Helicases/genetics,metabolism DNA Repair DNA, Circular/metabolism DNA, Single-Stranded/metabolism DNA, Viral/metabolism DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Kinetics Molecular Sequence Data Restriction Mapping Sequence Homology, Amino Acid Substrate Specificity
Chemicals
Bacterial Proteins DNA, Circular DNA, Single-Stranded DNA, Viral DNA-Binding Proteins Escherichia coli Proteins RuvB protein, Bacteria Holliday junction DNA helicase, E coli DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsaneva I R
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom.
Müller B
West S C
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.4.1315
Published
1993-02-15
Pages
1315-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45863
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.4.1315 PMC Full Text View journal details
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