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PMID: 8430094 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Stabilization of alpha-helical structures in short peptides via end capping.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 3 ·1993-02-01 ·Pages 838-42

Forood B, Feliciano EJ, Nambiar KP

Abstract

The alpha-helix-stabilizing effect of different amino acid residues at the helical termini of short peptides in aqueous solution has been determined. Several dodecapeptides containing alanine, asparagine, aspartate, glutamine, glutamate, and serine at the amino terminus and arginine, lysine, and alanine at the carboxyl terminus were synthesized, and the alpha-helical content of each peptide was measured by using circular dichroism spectroscopy. The trend in alpha-helix-inducing ability of these amino acids was found to be as follows: aspartate > asparagine > serine > glutamate > glutamine > alanine at the amino terminus and arginine > lysine > alanine at the carboxyl terminus. Our results agree with the Presta and Rose hypothesis [Presta, L. G. & Rose, G. D. (1988) Science 240, 1632-1641] on the role of end capping in helix stabilization.

MeSH Terms
Amino Acid Sequence Amino Acids/chemistry Circular Dichroism Cold Temperature Fluorides Hot Temperature Hydrogen-Ion Concentration Molecular Sequence Data Oligopeptides/chemistry,drug effects Potassium/pharmacology Potassium Compounds Protein Structure, Secondary Spectrophotometry, Ultraviolet Trifluoroethanol/pharmacology
Chemicals
Amino Acids Oligopeptides Potassium Compounds Trifluoroethanol potassium fluoride Fluorides Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Forood B
Department of Chemistry, University of California, Davis 95616.
Feliciano E J
Nambiar K P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.3.838
Published
1993-02-01
Pages
838-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45765
Subset
IM
Grants
NIGMS NIH HHS · R29 GM 39822 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.3.838 PMC Full Text View journal details
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