Cloning, characterization, and use in strain improvement of the Cephalosporium acremonium gene cefG encoding acetyl transferase.
A long open reading frame (ORF) closely linked to the Cephalosporium acremonium gene cefEF was identified by DNA sequencing. The cefEF gene encodes the enzyme involved in cephalosporin C (CPC) biosynthesis known as expandase/hydroxylase. Complementation of a C. acremonium cefG mutant, as well as expression of the gene in Aspergillus niger, showed this ORF to be the cefG gene, encoding cephalosporin C acetyltransferase, which catalyzes the last step in CPC biosynthesis. Analysis of transformants containing additional copies of this gene showed that a direct relationship exists between cefG copy number, cefG message levels, and CPC titers. This gene encodes an enzyme for what may be a rate-limiting step in CPC production.
13 references, click to expand
-
Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium.Nature. 1985 Nov 14-20;318(6042):191-4 PMID: 3903520
-
The Aspergillus nidulans npeA locus consists of three contiguous genes required for penicillin biosynthesis.EMBO J. 1990 Jan;9(1):279-87 PMID: 2403928
-
The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes.J Biol Chem. 1990 Sep 25;265(27):16358-65 PMID: 2129535
-
Molecular biology of penicillin and cephalosporin biosynthesis.Antimicrob Agents Chemother. 1990 Jun;34(6):943-8 PMID: 2203307
-
Characterization of the Cephalosporium acremonium pcbAB gene encoding alpha-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin biosynthetic genes and evidence of multiple functional domains.J Bacteriol. 1991 Apr;173(7):2354-65 PMID: 1706706
-
A yeast mitochondrial chelator-sensitive protease that processes cytoplasmically synthesized protein precursors: isolation from yeast and assay.Methods Enzymol. 1983;97:337-44 PMID: 6361476
-
Recombinant DNA in filamentous fungi: progress and prospects.Crit Rev Biotechnol. 1987;6(4):357-93 PMID: 3333340
-
Cloning and characterization of the acyl-coenzyme A: 6-aminopenicillanic-acid-acyltransferase gene of Penicillium chrysogenum.Gene. 1989 Nov 30;83(2):291-300 PMID: 2555269
-
The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus.J Bacteriol. 1990 Jul;172(7):3952-8 PMID: 1694525
-
Cloning and sequencing of the beta-lactam hydroxylase gene (cefF) from Streptomyces clavuligerus: gene duplication may have led to separate hydroxylase and expandase activities in the actinomycetes.J Bacteriol. 1991 Jan;173(1):398-400 PMID: 1987130
-
Efficient integrative transformation of Cephalosporium acremonium.Curr Genet. 1987;12(5):337-48 PMID: 2833362
-
Cloning and heterologous expression of the penicillin biosynthetic gene cluster from penicillum chrysogenum.Biotechnology (N Y). 1990 Jan;8(1):39-41 PMID: 1368505
-
Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase.J Bacteriol. 1989 Feb;171(2):754-60 PMID: 2644235
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
