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PMID: 8422968 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Unique phylogenetic position of Diplomonadida based on the complete small subunit ribosomal RNA sequence of Giardia ardeae, G. muris, G. duodenalis and Hexamita sp.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology ·Vol. 7 ·No. 1 ·1993-01-00 ·Pages 223-31

van Keulen H, Gutell RR, Gates MA, Campbell SR, Erlandsen SL, Jarroll EL, Kulda J, Meyer EA

Abstract

Complete small-subunit rRNA (SSU-rRNA) coding region sequences were determined for two species of the intestinal parasite Giardia: G. ardeae and G. muris, both belonging to the order Diplomonadida, and a free-living member of this order, Hexamita sp. These sequences were compared to published SSU-rDNA sequences from a third member of the genus Giardia, G. duodenalis (often called G. intestinalis or G. lamblia) and various representative organisms from other taxa. Of the three Giardia sequences analyzed, the SSU-rRNA from G. muris is the smallest (1432 bases as compared to 1435 and 1453 for G. ardeae and G. duodenalis, respectively) and has the lowest G+C content (58.9%). The Hexamita SSU-rRNA is the largest in this group, containing 1550 bases. Because the sizes of the SSU-rRNA are prokaryotic rather than typically eukaryotic, the secondary structures of the SSU-rRNAs were constructed. These structures show a number of typically eukaryotic signature sequences. Sequence alignments based on constraints imposed by secondary structure were used for construction of a phylogenetic tree for these four taxa. The results show that of the four diplomonads represented, the Giardia species form a distinct group. The other diplomonad Hexamita and the microsporidium Vairimorpha necatrix appear to be distinct from Giardia.

MeSH Terms

Animals Base Sequence DNA, Ribosomal/classification,genetics Diplomonadida/classification,genetics Giardia/classification,genetics Molecular Sequence Data Nucleic Acid Conformation Phylogeny RNA, Ribosomal, 18S/classification,genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid

Chemicals

DNA, Ribosomal RNA, Ribosomal, 18S

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

van Keulen H

Department of Biology, Cleveland State University, Ohio 44115.

Gutell R R

Gates M A

Campbell S R

Erlandsen S L

Jarroll E L

Kulda J

Meyer E A

Article Info

Journal

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Abbr.

FASEB J

ISSN

0892-6638

DOI

10.1096/fasebj.7.1.8422968

Published

1993-01-00

Pages

223-31

Language

English

Region

United States

NLM ID

8804484

Subset

Grants

NIGMS NIH HHS · GM 48207 · United States

Databases

GENBANK

K00421, K02202, K02641, M10932, M12676, M12677, M19500, M21774, M27607, M32703, M33639, M54897, V00348, X02995, X03235, X03450, X03948, X65063, X66042, X66043, X66045, X66046, X67167, Y00266, Z17210, Z17224, Z18319

External Links

PubMed source DOI: 10.1096/fasebj.7.1.8422968 View journal details

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Unique phylogenetic position of Diplomonadida based on the complete small subunit ribosomal RNA sequence of Giardia ardeae, G. muris, G. duodenalis and Hexamita sp.

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