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PMID: 8416893 Published · ppublish English Journal Article

Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis.

Journal of bacteriology ·Vol. 175 ·No. 1 ·1993-01-00 ·Pages 182-9

Stassi D, Donadio S, Staver MJ, Katz L

Abstract

In analyzing the region of the Saccharopolyspora erythraea chromosome responsible for the biosynthesis of the macrolide antibiotic erythromycin, we identified a gene, designated eryK, located about 50 kb downstream of the erythromycin resistance gene, ermE. eryK encodes a 44-kDa protein which, on the basis of comparative analysis, belongs to the P450 monooxygenase family. An S. erythraea strain disrupted in eryK no longer produced erythromycin A but accumulated the B and D forms of the antibiotic, indicating that eryK is responsible for the C-12 hydroxylation of the macrolactone ring, one of the last steps in erythromycin biosynthesis.

Related Genes
eryk
MeSH Terms
Amino Acid Sequence Bacterial Proteins Base Sequence Chromosome Mapping Consensus Sequence Cytochrome P-450 Enzyme System/classification,genetics Erythromycin/biosynthesis Genes, Bacterial/genetics Hydroxylation Mixed Function Oxygenases/genetics Molecular Sequence Data Mutagenesis, Insertional Oxygenases/classification,genetics Saccharopolyspora/enzymology,genetics Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Erythromycin Cytochrome P-450 Enzyme System Mixed Function Oxygenases Oxygenases EryK protein, Saccharopolyspora erythraea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stassi D
Corporate Molecular Biology, Abbott Laboratories, Abbott Park, Illinois 60064.
Donadio S
Staver M J
Katz L
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.1.182-189.1993
Published
1993-01-00
Pages
182-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196112
Subset
IM
Databases
GENBANK
L05776
External Links
PubMed source DOI: 10.1128/jb.175.1.182-189.1993 PMC Full Text View journal details
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