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PMID: 8406586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

B7/BB-1 is a leucocyte differentiation antigen on human dendritic cells induced by activation.

Immunology ·Vol. 79 ·No. 4 ·1993-08-00 ·Pages 616-20

Hart DN, Starling GC, Calder VL, Fernando NS

Abstract

Activation of a primary T-lymphocyte response requires additional signals apart from interaction of the T-cell receptor (TcR)/CD3 complex with major histocompatibility complex (MHC) antigens on the antigen-presenting cell. The CD28 antigen on T lymphocytes provides an important co-stimulatory signal to T lymphocytes and we therefore searched for the presence of its ligand, the B7/BB-1 antigen, on blood and tonsil dendritic cells (DC). Blood DC, prepared from peripheral blood mononuclear cells with a minimal period of in vitro culture, did not stain with the monoclonal antibody BB-1 using flow cytometry analysis. In contrast, tonsil DC stained weakly for B7/BB-1 compared to positive control cell lines. Polymerase chain reaction (PCR) was used to amplify a 605 base pair (bp) fragment from human B7/BB-1 mRNA and demonstrated significant amounts of B7/BB-1 mRNA in tonsil DC but no specific product was obtained from blood DC, confirming the surface-staining results. Weak expression of B7/BB-1 antigen was detected by immunofluorescence analysis following culture of blood DC with either interferon-gamma (IFN-gamma) or granulocyte-macrophage colony-stimulating factor (GM-CSF). These data support the concept that blood DC give rise to tissue and/or lymphoid DC, which acquire co-stimulatory ligands as a result of activation and/or differentiation.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, CD/immunology Base Sequence Dendritic Cells/immunology Granulocyte-Macrophage Colony-Stimulating Factor/immunology Humans Interferon-gamma/immunology Ligands Lymphocyte Activation Molecular Sequence Data Palatine Tonsil/immunology Polymerase Chain Reaction RNA, Messenger/immunology T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Antigens, CD Ligands RNA, Messenger Interferon-gamma Granulocyte-Macrophage Colony-Stimulating Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hart D N
Haematology Department, Christchurch Hospital, New Zealand.
Starling G C
Calder V L
Fernando N S
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29 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1993-08-00
Pages
616-20
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1421907
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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